Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

doi:10.1007/s00343-020-9304-5

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
	Zhang, Qingchun 1,3,4; Niu, Zhuang 1,2,3,4; Wang, Jinxiu 1,2,3,4; Liu, Chao 1,2,3,4; Kong, Fanzhou 1,3,4; Hu, Xiaokun 1,2,3,4; Zhao, Jiayu 1,2,3,4; Yu, Rencheng 1,2,3,4
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-05-09
页码	17
关键词	Phaeocystis globosa chloroplast DNA marker phylogeny
ISSN号	2096-5508
DOI	10.1007/s00343-020-9304-5
通讯作者	Yu, Rencheng(rcyu@qdio.ac.cn)
英文摘要	Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world. The morphological, physiological, and biochemical characteristics are different among geographic strains, which can not be distinguished with nuclear ribosomal DNA markers at present. Therefore, the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1-D2 and ITS regions, and three chloroplast intergenic spacers (petN-trnS1, trnM1-psbA, and rbcS-rpl27) were analyzed and compared among 13 strains of P. globosa isolated from the Pacific Ocean and Atlantic Ocean in this study. In addition, the nucleotide polymorphisms of 28S rDNA D1-D2, ITS, and rbcS-rpl27 regions were evaluated in two P. globosa strains. The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1-D2 region and ITS region, but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer. A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer, which had the strongest distinction to geographic strains compared to those of 28S rDNA D1-D2 and ITS regions. In the phylogenetic tree of rbcS-rpl27 intergenic spacer, the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P. globosa, and then formed an independent clade, while the other Atlantic strains and all of Pacific strains joined up to build the other clade. It was implied that at least two genetically distant populations of P. globosa existed in the Atlantic coastal regions. This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P. globosa, and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P. globosa.
资助项目	National Key Research and Development Plan Project ; Sino-Australian Centre for Healthy Coasts[2016YFE0101500] ; Science & Technology Basic Resources Investigation Program[2018FY100206] ; National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41776127]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000531220300004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167487]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yu, Rencheng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qingchun,Niu, Zhuang,Wang, Jinxiu,et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020:17.
APA	Zhang, Qingchun.,Niu, Zhuang.,Wang, Jinxiu.,Liu, Chao.,Kong, Fanzhou.,...&Yu, Rencheng.(2020).Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,17.
MLA	Zhang, Qingchun,et al."Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2020):17.