Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates

doi:10.1007/s10811-019-01809-6

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates
	Deng, Yunyan 1,2,3; Hu, Zhangxi 1,2,3; Chai, Zhaoyang 2,3; Tang, Ying Zhong 1,2,3
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2019-10-01
卷号	31 期号:5 页码:2969-2979
关键词	Dinoflagellate Harmful algal blooms (HABs) Proliferating cell nuclear antigen (PCNA) Resting cyst Scrippsiella trochoidea
ISSN号	0921-8971
DOI	10.1007/s10811-019-01809-6
通讯作者	Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
英文摘要	Proliferating cell nuclear antigen (PCNA), a co-factor of DNA polymerases delta and epsilon, is associated with active cell proliferation, particularly with the S phase of the eukaryotic cell cycle, and the expression of this gene has been therefore proposed as a molecular marker for estimating phytoplankton growth rate and even population dynamics. In this study, the full-length cDNA sequences of PCNA for nine dinoflagellate species (ten strains) were obtained, analyzed, and further characterized with the expression in response to alterations of growth stage and life cycle. All the ten genes have a 777 or 780 bp ORF and encode a protein of 258 or 259 amino acids, similar to PCNAs from vertebrates and plants. The deduced amino acid sequences include conservative motif characteristic to the PCNA gene family, implying conserved functions of PCNA among organisms of different taxa. Alignment analysis in combination with other sequences available in NCBI database exhibited high amino acid similarities (79.2-96.9%) among PCNAs from 17 dinoflagellate species. Phylogenetic tree derived from 37 PCNA sequences of dinoflagellates was consistent with the taxonomic affiliation of these taxa. Then, we used the cosmopolitan, toxic, and resting cyst-producing dinoflagellate Scrippsiella trochoidea as a representative of HAB-forming dinoflagellates to investigate PCNA (StPCNA) expression at different stages of growth and life cycle and the results of real-time quantitative PCR revealed clearly a stage-dependent pattern in the transcription of StPCNA. The transcripts dramatically reduced from an exponential to a stationary growth stage and then further significantly decreased from a stationary stage to dormant stage (resting cysts), a pattern in accordance with that previously reported from the dinoflagellates Alexandrium catenella and Prorocentrum donghaiense. In terms of its minimal implication, this work enhances the previous perception about the function and possible application of PCNA gene, and more importantly, our results indicated that PCNA is not silent during the dormant stage of dinoflagellates.
资助项目	National Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000493895800019
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164946]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,et al. Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(5):2969-2979.
APA	Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,&Tang, Ying Zhong.(2019).Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates.JOURNAL OF APPLIED PHYCOLOGY,31(5),2969-2979.
MLA	Deng, Yunyan,et al."Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates".JOURNAL OF APPLIED PHYCOLOGY 31.5(2019):2969-2979.