A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata

doi:10.1093/gigascience/giz089

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata
	Liu, Qin 2,3,4,5; Guo, Yunhai 2,3,4,5; Zhang, Yi 2,3,4,5; Hu, Wei 2,3,4,5,6; Li, Yuanyuan 2,3,4,5; Zhu, Dan 2,3,4,5; Zhou, Zhengbin 2,3,4,5; Wu, Jiatong 2,3,4,5; Chen, Nansheng 1,7,8; Zhou, Xiao-Nong 2,3,4,5
刊名	GIGASCIENCE
	2019-08-01
卷号	8 期号:8 页码:8
关键词	Triatoma rubrofasciata PacBio Sequel platform Hi-C chromosomal-level assembly comparative genomics RNA-Seq Iso-Seq
ISSN号	2047-217X
DOI	10.1093/gigascience/giz089
通讯作者	Chen, Nansheng(chennn@sfu.ca) ; Zhou, Xiao-Nong(xiaonongzhou1962@gmail.com)
英文摘要	Background: Triatoma rubrofasciata is a widespread pathogen vector for Chagas disease, an illness that affects approximately 7 million people worldwide. Despite its importance to human health, its evolutionary origin has not been conclusively determined. A reference genome for T. rubrofasciata is not yet available. Finding: We have sequenced the genome of a female individual with T. rubrofasciata using a single molecular DNA sequencing technology (i.e., PacBio Sequel platform) and have successfully reconstructed a whole-genome (680-Mb) assembly that covers 90% of the nuclear genome (757 Mb). Through Hi-C analysis, we have reconstructed full-length chromosomes of this female individual that has 13 unique chromosomes (2n = 24 = 22 + X1 + X2) with a contig N50 of 2.72 Mb and a scaffold N50 of 50.7 Mb. This genome has achieved a high base-level accuracy of 99.99%. This platinum-grade genome assembly has 12,691 annotated protein-coding genes. More than 95.1% of BUSCO genes were single-copy completed, indicating a high level of completeness of the genome. Conclusion: The platinum-grade genome assembly and its annotation provide valuable information for future in-depth comparative genomics studies, including sexual determination analysis in T. rubrofasciata and the pathogenesis of Chagas disease.
资助项目	National Key Research and Development Program of China[2016YFC1202000] ; National Science and Technology Project[2018ZX10101002] ; CAS Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000491875600004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164871]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Chen, Nansheng; Zhou, Xiao-Nong
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 2.Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China 3.Minist Hlth, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China 4.WHO Collaborating Ctr Trop Dis, Shanghai 200025, Peoples R China 5.Chinese Ctr Trop Dis Res, Shanghai 200025, Peoples R China 6.Fudan, Sch Life Sci, Dept Microbiol & Microbial Engn, Shanghai 200025, Peoples R China 7.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 8.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada
推荐引用方式 GB/T 7714	Liu, Qin,Guo, Yunhai,Zhang, Yi,et al. A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata[J]. GIGASCIENCE,2019,8(8):8.
APA	Liu, Qin.,Guo, Yunhai.,Zhang, Yi.,Hu, Wei.,Li, Yuanyuan.,...&Zhou, Xiao-Nong.(2019).A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata.GIGASCIENCE,8(8),8.
MLA	Liu, Qin,et al."A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata".GIGASCIENCE 8.8(2019):8.