Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification

doi:10.1016/j.ecoenv.2021.112235

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	Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification
	Wang, Xiudan 4; Li, Changmei 4; Jia, Zhihao 5; Xu, Tongxiao 4; Wang, Yilin 4; Sun, Mingzhu 4; Han, Shuhui 4; Wang, Xia 4; Qiu, Limei 1,2,3
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2021-07-01
卷号	217 页码:10
关键词	Ocean acidification Crassostrea gigas Apoptosis CgBcl-XL
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2021.112235
通讯作者	Wang, Xia(wxbiology@qust.edu.cn) ; Qiu, Limei(qiulimei@qdio.ac.cn)
英文摘要	Ocean acidification (OA) has posed formidable threats to marine calcifiers. In response to elevated CO2 levels, marine calcifiers have developed multiple strategies to survive, such as taking advantage of apoptosis, but its regulation mechanism remains largely unknown. Here, we used the Pacific oyster Crassostrea gigas as model to understand the apoptotic responses and regulation mechanism at short- (7 d) to long-term (56 d) CO2 exposure (pH = 7.50). The apoptosis of hemocytes was significantly induced after short-term treatment (7-21 d) but was suppressed under long-term CO2 exposure (42-56 d). Similarly, caspase-3 and caspase-9 were also increased post short-term exposure and fell back to normal levels after long-term exposure. These data together indicated diverse regulation mechanisms of apoptosis through different exposure periods. Through analysis of the B-cell lymphoma 2 (Bcl-2) family mitochondrial apoptosis regulators, we showed that only CgBcl-XL's expression kept at high levels after 42- and 56-day CO2 exposure. CgBcl-XL shared sequence, and structural similarity with its mammalian counterpart, and knockdown of CgBcl-XL in hemocytes via RNA interference promoted apoptosis. The protein level of CgBcl-XL was significantly increased after long-term CO2 exposure (28-56 d), and its distribution in hemocytes became more concentrated and dense. Therefore, CgBcl-XL serves as an essential antiapoptotic protein for tipping the balance of cell apoptosis, which may play a key role in survival under longterm CO2 exposure. These results reveal a potential adaptation strategy of oysters towards OA and the variable environment changes through the modulation of apoptosis.
资助项目	National Key Research and Development Program of China[2019YFD0901705] ; Ministry of Science and Technology of the People's Republic of China
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000649634600007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170983]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Xia; Qiu, Limei
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 5.Soochow Univ, Cambridge Suda Genom Resource Ctr, Suzhou 215123, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiudan,Li, Changmei,Jia, Zhihao,et al. Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,217:10.
APA	Wang, Xiudan.,Li, Changmei.,Jia, Zhihao.,Xu, Tongxiao.,Wang, Yilin.,...&Qiu, Limei.(2021).Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,217,10.
MLA	Wang, Xiudan,et al."Regulation of apoptosis by Pacific oyster Crassostrea gigas reveals acclimation strategy to CO2 driven acidification".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217(2021):10.