MiR8181 is involved in the cell growth regulation of Saccharina japonica

doi:10.1016/j.jplph.2021.153394

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	MiR8181 is involved in the cell growth regulation of Saccharina japonica
	Yang, Xiaoqi 1,2,3; Wang, Xiuliang 2,3; Yao, Jianting 2,3; Li, Wei 1,2,3; Duan, Delin 2,3
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2021-05-01
卷号	260 页码:11
关键词	Aureochrome Growth miR8181 Degradome analysis Macroalgae Saccharina japonica
ISSN号	0176-1617
DOI	10.1016/j.jplph.2021.153394
通讯作者	Duan, Delin(dlduan@qdio.ac.cn)
英文摘要	Aureochrome, a blue-light receptor specifically found in photosynthetic stramenopiles, plays an important role in algal growth and development. It holds a reversed effector?sensor topology for the reception of blue light, acting as a candidate of optogenetic tool in transcriptional regulation. However, the inner regulatory mechanism of aureochrome is still unclear. In this study, we explored the potential regulatory relationship between microRNAs (miRNAs) and mRNAs by small RNA, transcriptome and degradome sequencing in Saccharina japonica. Through screening miRNA-mRNA interaction networks at the whole-genome level, we found that 18 miRNAs perfectly paired with aureochrome. Among these screened miRNAs, miR8181 was negatively correlated with aureochrome5 with high credibility, exhibiting tissue-specific expression in sporophyte of S. japonica. Degradome analysis further revealed the exact cleavage site of miR8181 on aureochrome5, confirming their targeting relationship. For the 54 target genes of miR8181, nine genes that exhibited similar expression to that of aureochrome5 competed for the same binding site, thus establishing a competing endogenous RNA network. Functional enrichment of the target genes revealed that miR8181 was involved in the regulation of cell differentiation and development in S. japonica. Moreover, overexpression of miR8181 resulted in significant decreases in the cell growth rates of Phaeodactylum tricornutum, suggesting negative roles of miR8181 in regulating cell growth. Our study revealed that miR8181, the targeting miRNA of aureochrome5, played negative roles in cell growth and development.
资助项目	National Natural Science Foundation of China[31772848] ; Marine S &T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ05021] ; [2017YFE0130900]
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000642485200006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170898]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiaoqi,Wang, Xiuliang,Yao, Jianting,et al. MiR8181 is involved in the cell growth regulation of Saccharina japonica[J]. JOURNAL OF PLANT PHYSIOLOGY,2021,260:11.
APA	Yang, Xiaoqi,Wang, Xiuliang,Yao, Jianting,Li, Wei,&Duan, Delin.(2021).MiR8181 is involved in the cell growth regulation of Saccharina japonica.JOURNAL OF PLANT PHYSIOLOGY,260,11.
MLA	Yang, Xiaoqi,et al."MiR8181 is involved in the cell growth regulation of Saccharina japonica".JOURNAL OF PLANT PHYSIOLOGY 260(2021):11.