Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)

doi:10.1016/j.theriogenology.2021.01.001

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)
	Zhao, Haixia 1,2,3,4; Wang, Xueying 2,3,4; Du, Tengfei 1,2,3,4; Gao, Guang 1,2,3,4; Wu, Lele 1,2,3,4; Xu, Shihong 2,3,4; Xiao, Yongshuang 2,3,4; Wang, Yanfeng 2,3,4; Liu, Qinghua 2,3,4; Li, Jun 2,3,4
刊名	THERIOGENOLOGY
	2021-05-01
卷号	166 页码:83-89
关键词	Sperm storage Sebastes schlegelii Viviparous Ovarian crypt Sperm motility
ISSN号	0093-691X
DOI	10.1016/j.theriogenology.2021.01.001
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
英文摘要	Sebastes schlegelii is a typical viviparous teleost with six months sperm storage duration from November to April. In this study, spermatozoa morphological and physiological characteristics and sperm location in the female ovary were investigated by electron microscopy, computer-assisted sperm analyzer and histologic analysis, respectively. During copulation, we observed that spermatozoa in the testis had mature structure with rod-shaped head, a short midpiece, and a long flagellum. And further verified sperm swam freely at a high speed in the ovary fluid. After copulation, we only found swimming sperm in the ovary fluid at the early storage stage (November to December) and the majority of sperm were scattered randomly in the ovary cavity and partially concentrated in the crypt between the oocyte and stalk of follicle. Thereafter, the ovarian epithelium around the oocytes proliferated rapidly and wrapping spermatozoa outside of the follicular layer and formed a lot of crypts outside of the follicular layer which served as the sperm storage site until fertilization. The present findings would be useful for further understanding the mechanism of long-term sperm storage in viviparous teleost. ? 2021 Published by Elsevier Inc.
资助项目	National Natural Science Foundation of China[31802278] ; National Key R&D Program of China[2018YFD0901205] ; National Key R&D Program of China[2018YFD0901204] ; China Agriculture Research System[CARS-47] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0302-4] ; Marine S & T Fund of Shandong Province for Pilot Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; National Infrastructure of Fishery Germplasm Resource[2019DKA30470] ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060]
WOS研究方向	Reproductive Biology ; Veterinary Sciences
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000637206400010
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170504]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Haixia,Wang, Xueying,Du, Tengfei,et al. Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)[J]. THERIOGENOLOGY,2021,166:83-89.
APA	Zhao, Haixia.,Wang, Xueying.,Du, Tengfei.,Gao, Guang.,Wu, Lele.,...&Li, Jun.(2021).Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii).THERIOGENOLOGY,166,83-89.
MLA	Zhao, Haixia,et al."Sperm maturation, migration, and localization before and after copulation in black rockfish (Sebastes schlegelii)".THERIOGENOLOGY 166(2021):83-89.