Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin-Strongylocentyotus internedius

doi:10.1016/j.carbpol.2021.117701

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	Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin-Strongylocentyotus internedius
	Wang, Qing-Chi 1,2,3,4; Wei, Maosheng 1,2,3,4; Yue, Yang 1,2,3,4; Wu, Ning 1,2,3,4; Wang, Jing 1,2,3,4; Zhang, Quanbin 1,2,3,4
刊名	CARBOHYDRATE POLYMERS
	2021-04-15
卷号	258 页码:14
关键词	Strongylocentyotus internedius Glycogen Structural characterization Immunostimulatory activity RAW264.7 macrophage
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.117701
通讯作者	Zhang, Quanbin(qbzhang@qdio.ac.cn)
英文摘要	Sea urchin possesses both high nutritional and medicinal value. It contains diverse biological active polysaccharides. But there are few studies on its glycogen. In the current study, a glucan (MSGA) was separated from Strongylocentyotus internedius and purified by ion exchange and gel filtration column. Chemical analysis revealed that MSGA with 2.65 x 10(7) Da is made up entirely of glucose. The analysis of methylation, NMR and mass spectrum demonstrated that MSGA is a highly branched glycogen with alpha-(1 -> 4) linked gluconic backbone and branched at C-6 (one branch per five residues). In addition, MSGA showed good in vitro immunostimulatory activity via NF-kappa B and MAPKs pathways. It is considered that high degree of branching is necessary for its activity. However, the relationship between structure and immunostimulatory activity of natural glycogens is difficult to elucidate because the difference in their structural properties. Therefore, much more research is needed in this area.
资助项目	National Key R&D Program of China[2018YFD0901104] ; National Key R&D Program of China[2019YFD0900705]
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000620151600005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170132]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Quanbin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Dept Earth Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qing-Chi,Wei, Maosheng,Yue, Yang,et al. Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin-Strongylocentyotus internedius[J]. CARBOHYDRATE POLYMERS,2021,258:14.
APA	Wang, Qing-Chi,Wei, Maosheng,Yue, Yang,Wu, Ning,Wang, Jing,&Zhang, Quanbin.(2021).Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin-Strongylocentyotus internedius.CARBOHYDRATE POLYMERS,258,14.
MLA	Wang, Qing-Chi,et al."Structural characterization and immunostimulatory activity in vitro of a glycogen from sea urchin-Strongylocentyotus internedius".CARBOHYDRATE POLYMERS 258(2021):14.