Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase

doi:10.3390/toxins13010044

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase
	Yue, Yang 1; Yu, Huahua 1,2; Li, Rongfeng 1,2; Li, Pengcheng 1,2
刊名	TOXINS
	2021
卷号	13 期号:1 页码:14
关键词	jellyfish venom metalloproteinase oedema vascular permeability type IV collagen
DOI	10.3390/toxins13010044
通讯作者	Yue, Yang(yueyang@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要	Scyphozoan envenomation is featured as severe cutaneous damages due to the toxic effects of venom components released by the stinging nematocysts of a scyphozoan. However, the oedematogenic property and mechanism of scyphozoan venoms remain uninvestigated. Here, we present the oedematogenic properties of the nematocyst venom from Nemopilema nomurai (NnNV), a giant stinging scyphozoan in China, for the first time, using in vivo and in vitro models with class-specific inhibitors. NnNV was able to induce remarkable oedematogenic effects, including induction of significant oedema in the footpad and thigh of mouse, and increase in vascular permeability in the dorsal skin and kidney. Moreover, batimastat, a specific metalloproteinase inhibitor, could significantly reduce the Evan's blue leakage in the damaged organs and attenuate paw oedema after 12 h, but exerted no influence on NnNV-induced thigh oedema. These observations suggested a considerable contribution of NnNV metalloproteinase-like components to the increased vasopermeability, and the participation was strongly suggested to be mediated by destroying the integrity of the vascular basement membrane. Moreover, partial isolation combined LC-MS/MS profiling led to identification of the protein species Nn65 with remarkable metalloproteinase activity. This study contributes to the understanding of the effector components underlying the cutaneous damages induced by scyphozoan stings.
资助项目	National Key R&D Program of China[2019YFC0312605] ; National Key R&D Program of China[2017YFE0111100-04] ; National Natural Science Foundation of China[41776163] ; National Natural Science Foundation of China[41876164] ; Shandong Province Natural Science Foundation of China[ZR2019QD012]
WOS研究方向	Food Science & Technology ; Toxicology
语种	英语
出版者	MDPI
WOS记录号	WOS:000610769100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170032]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yue, Yang; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase[J]. TOXINS,2021,13(1):14.
APA	Yue, Yang,Yu, Huahua,Li, Rongfeng,&Li, Pengcheng.(2021).Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase.TOXINS,13(1),14.
MLA	Yue, Yang,et al."Topical Exposure to Nemopilema nomurai Venom Triggers Oedematogenic Effects: Enzymatic Contribution and Identification of Venom Metalloproteinase".TOXINS 13.1(2021):14.