Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01

doi:10.1007/s00343-020-0203-6

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01
	Yue, Yang 2; Yu, Huahua 2,3; Li, Rongfeng 2,3; Hu, Linfeng 1; Liu, Song 2,3; Xing, Rong'e 2,3; Li, Pengcheng 2,3
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2021-02-22
页码	10
关键词	sea anemone symbiotic fungi phenazine-1-carboxylic acid antimicrobial activity
ISSN号	2096-5508
DOI	10.1007/s00343-020-0203-6
通讯作者	Yu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要	Marine symbiotic fungi represent an intriguing source of discovery of novel secondary metabolites with various biological activities. Sea anemones are benthic marine invertebrates, however, the cultivable symbiotic fungi residing in the sea anemones are paid few attentions compared to those derived from their cnidarian counterparts. Here we show the identification of antimicrobial secondary metabolites from the sea anemone-derived symbiotic fungi. Out of five isolated fungal strains, only the strain SMA01 showed strong antimicrobial activities, which was assigned into the genus Emericella based on the morphological characteristics and the ITS sequencing. Media swift from liquid fermentation to solid rice medium presented little influence on its antibacterial activity. A chemical investigation of the ethyl acetate extract of the Emericella sp. SMA01 led to discovery of the primary antibiotic metabolite phenazine-1-carboxylic acid. The IC50 values of the phenazine-1-carboxylic acid against Phytophthora capsici, Gibberella zeae, and Verticillium dahliae were determined to be 23.26-53.89 mu g/mL. To the best of our knowledge, this was the first report of Emericella sp. in sea anemones. The current study may benefit understanding of the defensive chemical interactions between the symbiotic fungi and their host sea anemones.
资助项目	National Natural Science Foundation of China[41376004] ; National Natural Science Foundation of China[41406152] ; Key Research and Development Project of Shandong Province[2018GHY115008] ; Youth Innovation Promotion Association of CAS ; Scientific and Technological Innovation Project[2015ASKJ02]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000620409100003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169724]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yu, Huahua; Li, Pengcheng
作者单位	1.Henan Inst Sci & Technol, Sch Chem & Chem Engn, Xinxiang 453003, Henan, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2021:10.
APA	Yue, Yang.,Yu, Huahua.,Li, Rongfeng.,Hu, Linfeng.,Liu, Song.,...&Li, Pengcheng.(2021).Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10.
MLA	Yue, Yang,et al."Isolation and identification of antimicrobial metabolites from sea anemone-derived fungus Emericella sp. SMA01".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2021):10.