Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis
Bao, Chenchang1; Song, Chengwen2; Liu, Yuan2; Yang, Yanan1; Cui, Zhaoxia1,3
刊名JOURNAL OF PROTEOMICS
2020-08-30
卷号226页码:9
关键词Lysine crotonylation Proteomics Spermiogenesis Eriocheir sinensis
ISSN号1874-3919
DOI10.1016/j.jprot.2020.103891
通讯作者Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
英文摘要Lysine crotonylation (Kcr) is a recently-discovered type of post-translational modification. Although Kcr has been reported in many species, little is known about this process in crustaceans. In this study, pan anti-lysine crotonylation antibody enrichment and high-resolution liquid chromatogram-mass spectrometry analysis were employed to characterize Kcr in testis of the Chinese mitten crab Eriocheir sinensis testis. Overall, 2799 Kcr sites were identified on 908 proteins with 14 conserved motifs. Bioinformatics analysis showed that Kcr was predominant on proteins found in cytoplasm, mitochondria and nucleus, and those involved in ribosome, proteasome, carbon metabolism and protein processing in endoplasmic reticulum. In total, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. These differentially expressed proteins were enriched in protein processing in endoplasmic reticulum pathway during formation of acrosome. In contrast, histone Kcr associated with mammalian spermatogenesis. These results provide foundational knowledge on the role of non-histone Kcr in spermiogenesis of E. sinensis. Significance: Lysine crotonylation (Kcr) is a recently-identified post-translational modification, and histone Kcr was found to associate with mammalian spermatogenesis. However, crotonylation of non-histone proteins has not been reported in spermatogenesis regulation. Further, there is no information on crotonylation in crustaceans. This study was the first large-scale Kcr proteome characterization in crustaceans. A total of 2799 Kcr sites on 908 proteins with 14 conserved motifs were identified from Eriocheir sinensis testis. Of which, 83 up-regulated and 12 down-regulated non-histone crotonylated sites were identified during spermiogenesis. Our results provide the basic information for further functional validation of Kcr proteins and revealed new roles of Kcr in spermiogenesis of E. sinensis.
资助项目National Key R&D Program of China[2018YFD0900303] ; Ten Thousand Talents Program ; K. C. Wong Magna Fund in Ningbo University
WOS研究方向Biochemistry & Molecular Biology
语种英语
出版者ELSEVIER
WOS记录号WOS:000566934000008
内容类型期刊论文
源URL[http://ir.qdio.ac.cn/handle/337002/168749]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Cui, Zhaoxia
作者单位1.Ningbo Univ, Sch Marine Sci, Ningbo 315020, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
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GB/T 7714
Bao, Chenchang,Song, Chengwen,Liu, Yuan,et al. Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis[J]. JOURNAL OF PROTEOMICS,2020,226:9.
APA Bao, Chenchang,Song, Chengwen,Liu, Yuan,Yang, Yanan,&Cui, Zhaoxia.(2020).Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis.JOURNAL OF PROTEOMICS,226,9.
MLA Bao, Chenchang,et al."Large-scale lysine crotonylation analysis reveals its potential role in spermiogenesis in the Chinese mitten crab Eriocheir sinensis".JOURNAL OF PROTEOMICS 226(2020):9.
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