Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model

doi:10.1016/j.fsi.2020.05.063

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model
	Wu, Peiwen 2,3,4,5; Zhou, Shihui 2,3,4,5; Su, Zehui 2,3,4,5; Liu, Chang 2,3,4,5; Zeng, Fuyuan 2,3,4,5; Pang, Huanying 1,2,3,4,5,6; Xie, Miao 2,3,4,5; Jian, Jichang 1,2,3,4,5,6
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-09-01
卷号	104 页码:123-132
关键词	Vibrio alginolyticus vscQ Live attenuated vaccines T3SS
ISSN号	1050-4648
DOI	10.1016/j.fsi.2020.05.063
通讯作者	Pang, Huanying(phying1218@163.com) ; Jian, Jichang(jianjic@gdou.edu.cn)
英文摘要	Vibrio alginolyticus, a Gram-negative bacterium, has been recognized as an opportunistic pathogen in marine animals as well as humans. Type III secretion system (T3SS) is critical for pathogen virulence and disease development. However, no more information is known about the C-ring component VscQ and its physiological role. In this study, gene vscQ was cloned from V. alginolyticus wild-type strain HY9901 and the mutant strain HY9901 Delta vscQ was constructed by the in-frame deletion method. The HY9901 Delta vscQ mutant showed an attenuated swarming phenotype and a closely 4.6-fold decrease in the virulence to Danio rerio. However, the HY9901 Delta vscQ mutant showed no difference in growth, biofilm formation and ECPase activity. HY9901 Delta vscQ reduces the release of LDH, NO and caspase-3 activity of infected FHM cell, which are involved in fish cell apoptosis. Deletion of gene vscQ downregulates the expression level of T3SS-related genes including vscL, vopB, hop, vscO, vscK, vopD, vcrV and vopS and flagellum-related genes (flaA and fliG). And Danio rerio vaccinated via i.m injection with HY9901 Delta vscQ induced a relative percent survival (RPS) value of 71% after challenging with the wild-type HY9901. Real-time PCR assays showed that vaccination with HY9901 Delta vscQ enhanced the ex- pression of immune-related genes, including TNF-alpha, TLR5, IL-6R, IgM and c/ebp beta in liver and spleen after vaccination, indicating that it is able to induce humoral and cell-mediated immune response in zebrafish. These results demonstrate that the HY9901 Delta vscQ mutant could be used as an effective live vaccine to combat V. alginolyticus infection.
资助项目	Independent Project of Southern Marine Science and Engineering Guangdong Laboratory[ZJW-2019-05] ; National Natural Science Foundation of China[31402344] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; National Key R & Development Program of China[2018YFD0900501]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000564750000002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168510]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Pang, Huanying; Jian, Jichang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524025, Peoples R China 3.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China 4.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Peoples R China 5.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524025, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wu, Peiwen,Zhou, Shihui,Su, Zehui,et al. Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:123-132.
APA	Wu, Peiwen.,Zhou, Shihui.,Su, Zehui.,Liu, Chang.,Zeng, Fuyuan.,...&Jian, Jichang.(2020).Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model.FISH & SHELLFISH IMMUNOLOGY,104,123-132.
MLA	Wu, Peiwen,et al."Functional characterization of T3SS C-ring component VscQ and evaluation of its mutant as a live attenuated vaccine in zebrafish (Danio rerio) model".FISH & SHELLFISH IMMUNOLOGY 104(2020):123-132.