Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus

doi:10.1016/j.aquaculture.2020.735493

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus
	Liang, Shaoshuai 2,3; Wang, Wenxiang 1,2,3; Wang, Lijuan 2,3; Wu, Zhihao 2,3; Zou, Yuxia 2,3; Tan, Xungang 2,3; Liu, Yan 2,3; Peng, Zhuangzhuang 2,3; You, Feng 2,3
刊名	AQUACULTURE
	2020-11-15
卷号	528 页码:11
关键词	Figla Female-predominant expression RNAi Oocyte Olive flounder Paralichthys olivaceus
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2020.735493
英文摘要	Factor in the germ line alpha (a basic helix-loop-helix transcription factor, FIGLA) gene specifically expresses in female germ cells and is essential for ovarian differentiation and development in vertebrates. However, research on the roles in fish gonadal differentiation and development is limited. In this study, we cloned and characterized a figla gene from the olive flounder, Paralichthys olivaceus, an important marine cultured fish in China, Japan, and Korea. The full-length cDNA of the Po-figla is 1316 bp along with a 609 bp open reading frame. Real-time quantitative polymerase chain reaction analysis (qPCR) revealed that it was abundantly expressed in the ovary and female brain. During stages I-V of the ovary, the level of Po-figla mRNA reached the peak at stage II, and then gradually decreased, while Po-figla mRNA was slightly expressed along with the testicular development. Further, one color and two-color in situ hybridization results showed that the gene was mainly located in oocytes. Knocking down Po-figla in the ovary using RNAi led to its down-regulated expression and the reduction of oocyte number. In addition, qPCR results presented the differential expression patterns of Po-figla during gonadal differentiation period. In the exogenous 17 beta-estradiol treatment group (5 ppm), Po-figla expression levels constitutively increased in the differentiating and differentiated ovaries, while in the testosterone (5 ppm) treatment group, it was lower expressed in the testis during testicular differentiation period. Our findings provide evidence that Po-figla is involved in P. olivaceus ovarian differentiation and development, especially might have roles in the proliferation of fish oocytes. These findings provide the basic data for fish reproduction and gonadal development regulation in aquaculture.
资助项目	National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000553682600010
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/168096]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liang, Shaoshuai,Wang, Wenxiang,Wang, Lijuan,et al. Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus[J]. AQUACULTURE,2020,528:11.
APA	Liang, Shaoshuai.,Wang, Wenxiang.,Wang, Lijuan.,Wu, Zhihao.,Zou, Yuxia.,...&You, Feng.(2020).Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus.AQUACULTURE,528,11.
MLA	Liang, Shaoshuai,et al."Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus".AQUACULTURE 528(2020):11.