Characterization and Function Analysis of the Beta-Carotene Oxygenase-like Genes in Carotenoids Metabolism of the Ridgetail White PrawnExopalaemon carinicauda

doi:10.3389/fphys.2020.00745

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization and Function Analysis of the Beta-Carotene Oxygenase-like Genes in Carotenoids Metabolism of the Ridgetail White PrawnExopalaemon carinicauda
	Jin, Yue 1,2,3; Yu, Yang 1,2,4; Zhang, Chengsong 1,2,4; Li, Shihao 1,2,4; Zhang, Xiaojun 1,2,4; Li, Fuhua 1,2,4,5
刊名	FRONTIERS IN PHYSIOLOGY
	2020-07-09
卷号	11 页码:11
关键词	carotenoid oxygenases beta-carotene oxygenase-like genes carotene metabolism Exopalaemon carinicauda
ISSN号	1664-042X
DOI	10.3389/fphys.2020.00745
通讯作者	Yu, Yang(yuyang@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.ca)
英文摘要	Carotenoids are almost universally distributed in living organisms. The oxidative metabolism by carotene oxygenase contributes to the metabolic processes of carotenoids. 15,15MODIFIER LETTER PRIME-beta-carotene oxygenase (BCO1) and 9MODIFIER LETTER PRIME,10MODIFIER LETTER PRIME-beta-carotene oxygenase (BCO2) are two important carotenoid oxygenases. In order to understand the function of carotenoid oxygenases in crustaceans, seven genes encoding carotenoid oxygenases (namedEcBCO-like) were isolated from the transcriptome database ofExopalaemon carinicauda. After phylogenetic analysis with carotenoid oxygenases reported in other species,EcBCO-like1,EcBCO-like3, andEcBCO-like6were chosen for further functional study. The prawns afterEcBCO-like1knockdown suffered continuous death, which suggested its important role for the survival of the animals. For the prawns afterEcBCO-like3knockdown, no phenotype change was observed. The prawns afterEcBCO-like6knockdown showed color changes in their hepatopancreas when they were fed with carotenoids-containing diet, and the content of carotenoid in their hepatopancreas was much higher than that in the control prawns. The present study will pave the way for further understanding the carotenoids metabolism in the prawns.
资助项目	National Key R&D Program of China[2018YFD0900103] ; National Natural Science Foundation of China[31872552] ; China Agriculture Research System-48
WOS研究方向	Physiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000555581700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168087]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Yu, Yang; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Jin, Yue,Yu, Yang,Zhang, Chengsong,et al. Characterization and Function Analysis of the Beta-Carotene Oxygenase-like Genes in Carotenoids Metabolism of the Ridgetail White PrawnExopalaemon carinicauda[J]. FRONTIERS IN PHYSIOLOGY,2020,11:11.
APA	Jin, Yue,Yu, Yang,Zhang, Chengsong,Li, Shihao,Zhang, Xiaojun,&Li, Fuhua.(2020).Characterization and Function Analysis of the Beta-Carotene Oxygenase-like Genes in Carotenoids Metabolism of the Ridgetail White PrawnExopalaemon carinicauda.FRONTIERS IN PHYSIOLOGY,11,11.
MLA	Jin, Yue,et al."Characterization and Function Analysis of the Beta-Carotene Oxygenase-like Genes in Carotenoids Metabolism of the Ridgetail White PrawnExopalaemon carinicauda".FRONTIERS IN PHYSIOLOGY 11(2020):11.