The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas

doi:10.3389/fmars.2020.00399

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas
	Ding, Fangfang 2,6; Li, Ao 2,3,4,6; Cong, Rihao 1,6; Wang, Xinxing 2,6; Wang, Wei 3,5,6; Que, Huayong 1,6; Zhang, Guofan 1,4,5,6; Li, Li 3,4,5,6
刊名	FRONTIERS IN MARINE SCIENCE
	2020-06-30
卷号	7 页码:15
关键词	Pacific oyster artificial selection thermotolerance genetic structure physiological traits
DOI	10.3389/fmars.2020.00399
通讯作者	Zhang, Guofan(gfzhang@qdio.ac.cn) ; Li, Li(lili@qdio.ac.cn)
英文摘要	Investigating responses of organisms to stressful or new environments with selection pressure is one of the crucial problems in evolutionary biology, and it is of importance to understand the phenotypic and molecular mechanism underlying thermal tolerance under the context of the climate change. The Pacific oyster, Crassostrea gigas, inhabiting the environment with high variation in temperature, is a worldwide aquaculture species. However, summer mortality relevant to the high temperature is one of the problems challenging the oyster industry. An artificial selective breeding program was initiated to select for the thermal tolerance of oysters in an attempt to increase the summer survival rates since 2017 in our study. Parents of thermotolerance oyster selection is are based on acute thermal tolerance under controlled heat stress to strengthen the selection intensity. Furthermore, the phenotypic and the genotypic response to extreme high temperature were investigated based on the comparison of the F-1 progeny of the selected and natural populations in growth, physiology, mortality rate post heat stress, genetic structure, and gene expression. The parameter of growth showed no significant change between the selected and natural populations for the 6-month-old oysters. The selected population exhibited a higher survival rate after exposure to heat stress in the laboratory, which is in line with result of the filed experience that summer mortality of selected population was significantly lower than that of natural population. Further, the respiration rate of the selected population increased at 38 degrees C, while it increased at 35 degrees C in the natural population. Simultaneously, metabolism-related enzymes (PK, SOD) showed higher activity levels in the selected population. Furthermore, phylogenetic analysis, population structure, and principal component analysis (PCA) suggested that the selected and natural populations exhibited genetic divergence, with eight genes (IF4A2, IF6, EIF3A, MANBA, DDX43, RECS, CAT2, and BAG4) in the selected regions showing differential expression patterns in response to heat stress in the two populations. This study suggests that artificial selection has a significant effect on phenotype and genome structure for the oyster, our study providing an alternative way to reveal the mechanism underlying thermotolerance that plays an important role to predict the potential adaptation to the climate change.
资助项目	National Key R&D Program of China[2018YFD0900304] ; China Postdoctoral Science Foundation[2019TQ0324] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q06] ; Technology and the Modern Agro-Industry Technology Research System[CARS-49]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000548180400001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167948]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Guofan; Li, Li
作者单位	1.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Key Lab Ecol Mariculture, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Ding, Fangfang,Li, Ao,Cong, Rihao,et al. The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas[J]. FRONTIERS IN MARINE SCIENCE,2020,7:15.
APA	Ding, Fangfang.,Li, Ao.,Cong, Rihao.,Wang, Xinxing.,Wang, Wei.,...&Li, Li.(2020).The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas.FRONTIERS IN MARINE SCIENCE,7,15.
MLA	Ding, Fangfang,et al."The Phenotypic and the Genetic Response to the Extreme High Temperature Provides New Insight Into Thermal Tolerance for the Pacific Oyster Crassostrea gigas".FRONTIERS IN MARINE SCIENCE 7(2020):15.