PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan

doi:10.1007/s00343-019-9013-0

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
	Yang Yue 1,2,3; Xing Rong'e 2,3; Liu Song 2,3; Qin Yukun 2,3; Li Kecheng 2,3; Yu Huahua 2,3; Li Pengcheng 2,3
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-05-01
卷号	38 期号:3 页码:834-840
关键词	hydroxypropyltrimethyl ammonium chloride chitosan RAW 264 7 cells PI3K Akt pathway nuclear factor-kappa B activating protein 1
ISSN号	2096-5508
DOI	10.1007/s00343-019-9013-0
通讯作者	Xing Rong'e(xingronge@qdio.ac.cn) ; Li Pengcheng()
英文摘要	We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) promoted the production of nitric oxide (NO) and proinflammatory cytokines by activating the mitogen-activated protein kinases (MAPK) and Janus kinase (JAK)/STAT pathways in RAW 264.7 cells, indicating good immunomodulatory activity of HACC. In this study, to further investigate the immunomodulatory mechanisms of HACC, we determined the roles of phosphatidylinositol 3-kinase (PI3K)/Akt, activating protein (AP-1) and nuclear factor kappa B (NF-kappa B) in HACC-induced activation of RAW 264.7 cells by the western blotting. The results suggest that HACC promoted the phosphorylation of p85 and Akt. Furthermore, c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC, indicating the translocation of NF-kappa B and AP-1 from cytoplasm to nucleus. In addition, as scanning electron microscopy (SEM) analysis shows, the cell morphology changed after HACC treatment. These findings indicate that HACC activated MAPK, JAK/STAT, and PI3K/Akt signaling pathways dependent on AP-1 and NF-kappa B activation in RAW 264.7 cells, ultimately leading to the increase of NO and cytokines.
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000536606000021
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167545]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Xing Rong'e; Li Pengcheng
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Yang Yue,Xing Rong'e,Liu Song,et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(3):834-840.
APA	Yang Yue.,Xing Rong'e.,Liu Song.,Qin Yukun.,Li Kecheng.,...&Li Pengcheng.(2020).PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(3),834-840.
MLA	Yang Yue,et al."PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.3(2020):834-840.