Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions

doi:10.1016/j.dci.2020.103689

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions
	Sun, Mingzhe 1,2; Li, Shihao 1,2,3; Zhang, Xiaojun 1,2,3; Xiang, Jianhai 1,2; Li, Fuhua 1,2,3
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2020-07-01
卷号	108 页码:12
关键词	Hemocytes subpopulation Transcriptome Phagocytosis Macropinocytosis Penaeid shrimp
ISSN号	0145-305X
DOI	10.1016/j.dci.2020.103689
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	Hemocytes in shrimp play important roles in innate immune responses against pathogens. Although three types of hemocytes including hyalinocytes, semi-granulocytes and granulocytes were identified based on their morphological characters in penaeid shrimp, knowledge about the molecular basis of their functions in the immunity is still very limited. In the present study, three subpopulations of hemocytes were firstly separated by Percoll gradient centrifugation, and their transcriptomes were analyzed. The data showed that significantly differential gene expression patterns existed in different types of hemocytes. The genes encoding phagocytic receptors, lectins and actin cytoskeleton involved in phagocytosis were highly expressed in hyalinocytes, while genes involved in the humoral immunity signaling pathways were highly expressed in semi-granulocytes, and genes encoding prophenoloxidase (proPO)-activating enzyme and serine proteases involved in proPO system activation were highly expressed in granulocytes. Further flow cytometry analysis indicated that hyalinocytes were the main hemocytes subpopulation responsible for ingesting foreign fluorescent beads, and this ingestion process mainly depends on the endocytic way of macropinocytosis. These data provide valuable information for understanding the molecular basis of distinct shrimp hemocytes subpopulations of shrimp in cellular and humoral immunity.
资助项目	Major Science and Technology Innovation Projects in Shandong Province[2018SDKJ0302-5] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48[CARS-48]
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000527338800013
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167190]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Mingzhe,Li, Shihao,Zhang, Xiaojun,et al. Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,108:12.
APA	Sun, Mingzhe,Li, Shihao,Zhang, Xiaojun,Xiang, Jianhai,&Li, Fuhua.(2020).Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,108,12.
MLA	Sun, Mingzhe,et al."Isolation and transcriptome analysis of three subpopulations of shrimp hemocytes reveals the underlying mechanism of their immune functions".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 108(2020):12.