An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study

doi:10.1186/s40543-020-00210-0

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study
	Li, Xiuxue 1,4,5,6; Wang, Lijun 1,4,5,6; Jia, Xiaoling 1,4,5,6; Guo, Chuanlong 1,3,4,5,6; Li, Chao 1,4,5,6; Zhang, Jiajia 1,4,5,6; Shi, Dayong 1,2,4,5,6
刊名	JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
	2020-04-06
卷号	11 期号:1 页码:7
关键词	Novel compound Quantitation UHPLC-TOF-MS Pharmacokinetics
ISSN号	2093-3134
DOI	10.1186/s40543-020-00210-0
通讯作者	Shi, Dayong(shidayong@sdu.edu.cn)
英文摘要	Background Bromophenol-thiosemicarbazone hybrid was a novel synthetic brominated anticancer compound with two bromine atoms. Bromophenol-thiosemicarbazone hybrid showed considerable selective inhibitory activity against PARP1 (IC50 = 29.5 nmol/L). UHPLC-TOF-MS was used to establish a new method to quantify bromophenol-thiosemicarbazone hybrid bio-samples for indispensable quantitation analysis in further extensive pre-clinical studies. Methods Chromatographic and mass spectrometry parameters were optimized for quantitative method establishment. Improved protein-precipitated method was applied to the extraction of bromophenol-thiosemicarbazone hybrid in rat plasma samples. Furthermore, this proposed method was applied to an intravenous bolus dose to male rats. Results Mobile phase was consisted of water for A and acetonitrile for B with 25 mmol/L formic acid in both A and B. The flow rate was 0.30 mL/min, and the run time of bromophenol-thiosemicarbazone hybrid was 4.0 min. A Thermo Fisher Accucore 2.6 mu m C18 column (50 x 2.1 mm i.d.; San Jose, USA) was used for chromatographic separation. High resolution mass spectrometry was used to quantify samples by exact mass number of compound which was operated on negative ionization mode. Linear dynamic range of the established method was widely with 13.7-10000 nmol/L. Pharmacokinetics properties of bromophenol-thiosemicarbazone hybrid were shown in the results. Conclusion This method was reliable and reproducible from sample preparation to analysis and storage stability under the investigated conditions. It may be useful for analysis of halogenated compounds and brominated compounds in ultra-performance liquid chromatography-mass spectrometry.
资助项目	National Natural Science Foundation of China[81773586] ; National Natural Science Foundation of China[81872906] ; National Natural Science Foundation of China[81703354] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-DQC014] ; Shandong Provincial Natural Science Foundation for Distinguished Young Scholars[JQ201722] ; NSFC-Shandong Joint Fund Project[U1706213]
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER INTERNATIONAL PUBLISHING AG
WOS记录号	WOS:000523574700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167093]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Shi, Dayong
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 2.Shandong Univ, State Key Lab Microbial Technol, 72 Binhai Rd, Qingdao 250100, Shandong, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Chem Engn, Dept Pharm, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiuxue,Wang, Lijun,Jia, Xiaoling,et al. An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study[J]. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,2020,11(1):7.
APA	Li, Xiuxue.,Wang, Lijun.,Jia, Xiaoling.,Guo, Chuanlong.,Li, Chao.,...&Shi, Dayong.(2020).An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study.JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY,11(1),7.
MLA	Li, Xiuxue,et al."An UHPLC-TOF-MS method for quantifying novel brominated anticancer compound bromophenol-thiosemicarbazone hybrid and its application to in vivo pharmacokinetic study".JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY 11.1(2020):7.