TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis

doi:10.1016/j.fsi.2019.11.039

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis
	Wang, Di 1,2; Zhang, Shujing 1; Liu, Baozhong 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-03-01
卷号	98 页码:1017-1023
关键词	TAF5L MITF Innate immunity Meretrix petechialis
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.11.039
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
英文摘要	TAF5L is a component of the P300/CBP-associated factor (PCAF) histone acetylase complex, which serves as a coactivator and takes part in basal transcription such as promoter recognition, complex assembly and transcription initiation. In our study, the full-length sequence of MpTAF5L was identified and characterized in the clam M. petechialis. Sequence analysis showed that the predicted MpTAF5L protein had a N-terminal TAF5-NTD2 domain and a C-terminal WD40-repeats domain. The annotation and evolutionary analysis revealed MpTAF5L had close evolutionary relationship with other invertebrate species. Tissue distribution analysis of TAFSL claimed that it was highly expressed in the mantle, adductor muscle, foot and hepatopancreas. The mRNA expression of MpTAF5L was significantly up-regulated after Vibrio parahaemolyticus challenge, indicating its involvement in the immune response of clam. Yeast two-hybrid assays verified that MpTAF5L can interact with MpMITF (a critical immune-related transcription factor), and our further research clarified this interaction depended upon the N-terminal TAF5-NTD2 domain of MpTAF5L. Moreover, the mRNA expression of MpBcl-2 (a target gene of MITF) was significantly decreased but the mRNA expression of MpMITF was not significantly changed after knockdown of MpTAF5L, which indicated the reduction of MpMITF regulating activity at the same time. These results revealed that MpTAF5L interacted with MpMITF and enhanced the activation of MpMITF, which plays roles in the immune defense against V. parahaemolyticus.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; China Agriculture Research System[CARS-49] ; National Natural Science Foundation of China[31772845]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000516880900107
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167041]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
推荐引用方式 GB/T 7714	Wang, Di,Zhang, Shujing,Liu, Baozhong. TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:1017-1023.
APA	Wang, Di,Zhang, Shujing,&Liu, Baozhong.(2020).TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,98,1017-1023.
MLA	Wang, Di,et al."TAF5L functions as transcriptional coactivator of MITF involved in the immune response of the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 98(2020):1017-1023.