Gene network analysis reveals a core set of genes involved in the immune response of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum infection

doi:10.1016/j.fsi.2019.11.033

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Gene network analysis reveals a core set of genes involved in the immune response of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum infection
	Ning, Xianhui 1,2; Sun, Li 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-03-01
卷号	98 页码:800-809
关键词	Paralichthys olivaceus Vibrio anguillarum Transcriptome Immunity WGCNA
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.11.033
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	Japanese flounder (Paralichthys olivaceus) is one of the most economically important marine fish cultured in north Asia. Vibrio anguillarum is a severe bacterial pathogen to Japanese flounder and many other aquaculture species. In order to understand the immune response of flounder during bacterial infection, we systematically examined the transcriptome profiles of flounder spleen at three time points after V. anguillarum challenge. More than one billion high quality reads were obtained, approximately 80.70% of which were successfully mapped to the reference genome of flounder. A total of 6060, 4688 and 4235 differentially expressed genes (DEGs) were captured at 6, 12 and 24-h post-infection, respectively. The DEGs exhibited dynamic changes in expression and were assigned into four different profiles based on expression trend. GO and KEGG analysis showed that the DEGs were enriched in various immune-related terms, including response to stimulation, immune system and pathways of cytokine-cytokine receptor interaction, Jak-STAT signaling and Toll-like receptor signaling. Furthermore, a network of highly interactive DEGs involved in 11 immune-related pathways was detected by utilizing the weighted co-expressing network analysis (WGCNA). Accordingly, 26 hub genes were discovered that constituted an elaborate immune regulatory network and functioned mainly in pathogen recognition, antigen processing, and molecular signaling. The results of this study provided the first systematical transcriptome profile of flounder in association with V. anguillarum infection and can serve as a valuable resource of target genes for future studies on the molecular mechanisms underlying the immune defense of flounder against bacterial infection.
资助项目	National Key Research and Development Project of China[2018YFD0900500] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; National Natural Science Foundation of China[31730100] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000516880900084
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167023]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Ning, Xianhui,Sun, Li. Gene network analysis reveals a core set of genes involved in the immune response of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:800-809.
APA	Ning, Xianhui,&Sun, Li.(2020).Gene network analysis reveals a core set of genes involved in the immune response of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum infection.FISH & SHELLFISH IMMUNOLOGY,98,800-809.
MLA	Ning, Xianhui,et al."Gene network analysis reveals a core set of genes involved in the immune response of Japanese flounder (Paralichthys olivaceus) against Vibrio anguillarum infection".FISH & SHELLFISH IMMUNOLOGY 98(2020):800-809.