Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis

doi:10.3389/fmars.2020.00094

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis
	Wang, Lijuan 1,2; Wu, Zhihao 1,2; Wang, Yingjun 3; Liu, Mengxia 3; Song, Aihuan 3; Liu, Hongjun 3; You, Feng 1,2
刊名	FRONTIERS IN MARINE SCIENCE
	2020-02-27
卷号	7 页码:11
关键词	Sebastes schlegelii mitochondrial DNA microsatellite DNA genetic variation stock enhancement Lidao Bay
DOI	10.3389/fmars.2020.00094
通讯作者	You, Feng(youfeng@qdio.ac.cn)
英文摘要	The black rockfish Sebastes schlegelii is a commercially important fish species for marine fishery stock enhancement in Asia. This work aimed to evaluate the potential genetic impacts of releasing hatchery-reared juvenile black rockfish on wild stock in Lidao Bay, China. A partial sequence of the mitochondrial DNA (mtDNA) control region and 38 microsatellite DNA loci were used to assess the genetic impact. The haplotype diversity parameter (h) and nucleotide diversity parameter (pi) in hatchery-released stock were 0.902 and 0.00483, respectively. The h values in wild stock before stock enhancement and the mixed stock after enhancement were 0.970 and 0.939 (p = 0.025), respectively, and the pi values were 0.00581 and 0.00526 (p = 0.150), respectively. The mean effective number of alleles (Ae) in hatchery-released stock was 4.76, the mean polymorphism information content (PIC) was 0.674, the observed heterozygosity (Ho) was 0.668, and the expected heterozygosity (He) was 0.697. In wild stock before stock enhancement and the mixed stock after enhancement, the Ae, PIC, Ho, and He values were 6.01 and 5.82, 0.698 and 0.716, 0.709 and 0.741, and 0.735 and 0.754, respectively. These results indicated no marked decrease in the hatchery-released stock, although it displayed slightly lower levels of genetic diversity and heterozygosity than the wild stock. And the mixed stock after release exhibited similar genetic diversity to that of the wild stock before release. Accordingly, we propose that stock enhancement may not cause genetic diversity reduction on wild S. schlegelii stock in Lidao Bay over the short term. Whereas, discriminant analysis of principal components (DAPC) analysis identified three major clusters. The fixation index (F-ST) and analysis of molecular variance (AMOVA) also clearly showed low level but significant differentiations between the hatchery-released and wild stocks, and the wild stock before enhancement and the mixed stock after enhancement. Consequently, long-term genetic evaluation might be required.
资助项目	Major Agricultural Application Technology and Innovation Projects of Shandong Province (2017-2020) ; Special Program for Basic Research of the Ministry of Science and Technology of China[2014FY110500] ; National Infrastructure of Fishery Germplasm Resource[2017DKA30470]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000517573800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/165628]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Marine Biol Inst Shandong Prov, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lijuan,Wu, Zhihao,Wang, Yingjun,et al. Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis[J]. FRONTIERS IN MARINE SCIENCE,2020,7:11.
APA	Wang, Lijuan.,Wu, Zhihao.,Wang, Yingjun.,Liu, Mengxia.,Song, Aihuan.,...&You, Feng.(2020).Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis.FRONTIERS IN MARINE SCIENCE,7,11.
MLA	Wang, Lijuan,et al."Genetic Assessment of a Black Rockfish, Sebastes schlegelii, Stock Enhancement Program in Lidao Bay, China Based on Mitochondrial and Nuclear DNA Analysis".FRONTIERS IN MARINE SCIENCE 7(2020):11.