Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)

doi:10.1016/j.ygcen.2019.113291

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)
	Ji, Li-Qin 1,2; Rao, Ying-Zhu 1,3; Zhang, Yong 4; Chen, Rong 3; Tao, Ya-Xiong 1
刊名	GENERAL AND COMPARATIVE ENDOCRINOLOGY
	2020
卷号	285 页码:11
关键词	Epinephelus coioides Orange-spotted grouper Melanocortin-1 receptor Melanocortin receptor accessory protein 2 Tissue distribution Pharmacology
ISSN号	0016-6480
DOI	10.1016/j.ygcen.2019.113291
通讯作者	Tao, Ya-Xiong(taoyaxi@auburn.edu)
英文摘要	Melanocortin-1 receptor (MC1R) has important roles in regulating pigmentation and inflammation. Melanocortin receptor accessory protein 2 (MRAP2) modulates trafficking, ligand binding, and signaling of mammalian melanocortin receptors. However, the effect of MRAP2 on fish MC1R has not been extensively studied. Herein, we cloned the orange-spotted grouper (Epinephelus coioides) mc1r, which had a 972 bp open reading frame encoding a putative protein of 323 amino acids. Grouper mclr was mainly expressed in the brain, skin, testis, spleen, head kidney, and kidney. EcoMC1R showed high constitutive activities in both Gs-cAMP and ERK1/2 pathways, which could be differentially modulated by grouper MRAP2 (EcoMRAP2). Three agonists, including alpha-melanocyte-stimulating hormone (MSH), beta-MSH, and ACTH, could bind to EcoMC1R and dose-dependently increase intracellular cAMP production. EcoMRAP2 had no effect on the IC50 in binding assay or EC50 in cAMP assay; however, it dose-dependently decreased the cell surface expression and maximal response to the three agonists. EcoMRAP2 increased basal ERK1/2 activation but did not alter alpha-MSH-stimulated ERK1/2 activation. This study extends the knowledge base of fish MC1R pharmacology and its regulation by MRAP2.
资助项目	Science and Technology Planning Project of Guangdong Province[2014A020208136] ; Science and Technology Planning Project of Zhanjiang[2018A03027] ; Key Science Project of Lingnan Normal University[LZL1508] ; Guangdong Provincial Natural Science Foundation[20188030311026] ; Guangdong Provincial Science and Technology Program[2017B0202450001] ; Guangdong Provincial Science and Technology Program[2017B090904022] ; Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams ; Special Fund for Fisheries -Scientific Research of Guangdong Province[SDYY-2018-04] ; Science and Technology Planning Project of Guangzhou[201804020013] ; Independent Project of Guangdong Province Laboratory[ZJW-2019-06] ; Overseas Scholarship Program for Elite Young and Middle-aged Teachers of Lingnan Normal University ; Auburn University Animal Health and Disease Research Program of College of Veterinary Medicine at Auburn University ; China Scholarship Council, People's Republic of China
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000504341800020
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164130]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Tao, Ya-Xiong
作者单位	1.Auburn Univ, Coll Vet Med, Dept Anat Physiol & Pharmacol, Auburn, AL 36849 USA 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Lingnan Normal Univ, Life Sci & Technol Sch, Inst Appl Biotechnol, Zhanjiang 524048, Guangdong, Peoples R China 4.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Southern Lab Ocean Sci & Engn Zhuhai, Zhuhai 51900, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,et al. Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2020,285:11.
APA	Ji, Li-Qin,Rao, Ying-Zhu,Zhang, Yong,Chen, Rong,&Tao, Ya-Xiong.(2020).Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides).GENERAL AND COMPARATIVE ENDOCRINOLOGY,285,11.
MLA	Ji, Li-Qin,et al."Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 285(2020):11.