Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation

doi:10.1016/j.chemosphere.2019.124935

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	Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation
	Liu, Yan 1,2; Wang, Lihong 1; Zhu, Long 1; Ran, Benhui 1; Wang, Zaizhao 1
刊名	CHEMOSPHERE
	2020-02-01
卷号	240 页码:10
关键词	Epigenetic modification Gobiocypris rarus Bisphenol A Steroid hormones Ovarian development
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2019.124935
通讯作者	Wang, Zaizhao(zzwang@nwsuaf.edu.cn)
英文摘要	Increasing studies have established the toxic effects of BPA on development and reproduction in animals. In present study, we investigated epigenetic effects on the transcription of several ovarian steroidogenic genes in rare minnows Gobiocypris rarus after BPA exposure at 15 mu gL(-1) for 21, 42 and 63 d. Results showed that short term BPA exposure (21 d) caused significant increase of both estradiol and testerone levels whereas long term exposure (63 d) led to significant decrease of them. The oocytes development was hindered after BPA exposure. BPA treatments for 21 and 42 d resulted in significant increase of genome DNA methylation in ovary while 63-d exposure caused marked decrease. The histone trimethylation levels (H3K4me3, H3K9me3 and H3K27me3) in the ovary were also disturbed by BPA. H3K9me3 was significantly decreased after 21 d whereas it was markedly increased after 42 and 63 d. The 42-d exposure caused significant decrease for H3K4me3. Meanwhile, 42- and 63-d BPA exposure led to significant decrease of H3K27me3. DNA methylation could involve in gene expression regulation of cypl7al and cypl9ala after BPA exposure. After short (21 d) and long term (63 d) BPA exposure, the respective mRNA expression down-regulation and up-regulation of star, cypllal, and cypl7al were mediated by H3K9me3. This study suggests that epigenetic modulation including DNA and histone methylation could be responsible for the detrimental effects on ovary development upon BPA exposure in G. rams. It is speculated that BPA exposures for short or long term duration could disturb the steroidogenesis in entirely different mechanisms. (C) 2019 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[31470553]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000497600800060
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163768]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Zaizhao
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yan,Wang, Lihong,Zhu, Long,et al. Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation[J]. CHEMOSPHERE,2020,240:10.
APA	Liu, Yan,Wang, Lihong,Zhu, Long,Ran, Benhui,&Wang, Zaizhao.(2020).Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation.CHEMOSPHERE,240,10.
MLA	Liu, Yan,et al."Bisphenol A disturbs transcription of steroidogenic genes in ovary of rare minnow Gobiocypris rarus via the abnormal DNA and histone methylation".CHEMOSPHERE 240(2020):10.