Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa : Subcellular localization and role in triacylglycerol synthesis

doi:10.1016/j.algal.2020.102172

CORC > 海洋研究所 > 中国科学院海洋研究所

	Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa : Subcellular localization and role in triacylglycerol synthesis
	Sun, Li-Ping 1,4,5; Ouyang, Long-Ling 1,3,4,5; Bao, Hong 1,4,5; Liu, Jian-Guo 2; Sun, Zheng 1,4,5; Zhou, Zhi-Gang 1,4,5
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2021-04-01
卷号	54 页码:10
关键词	Myrmecia incisa Molecular characterization Immunoelectron microscopy GFP fusion Enzyme activity
ISSN号	2211-9264
DOI	10.1016/j.algal.2020.102172
通讯作者	Sun, Zheng(zsun@shou.edu.cn) ; Zhou, Zhi-Gang(zgzhou@shou.edu.cn)
英文摘要	Microalgae represent a rich and naturally occurring source of triacylglycerol (TAG), and the microalgae-derived TAG has enormous potential in bioenergy, bio-based materials and other agricultural innovations. Glycerol-3-phosphate acyltransferase (GPAT) is a key enzyme that catalyzes the biosynthesis of TAG. Previously our lab has reported the first GPAT in the oleaginous alga Myrmecia incisa, and in the present study, a new GPAT was further identified from the same alga, which was designated as MiGPAT2. A full-length cDNA of MiGPAT2 consisting of a 1374-bp ORF, a 153-bp 5'-UTR and a 271-bp 3' -UTR was cloned. The putative protein composed of 457 amino acids, possessing the HX4D motif that acts as the activity center of GPAT catalysis. Emphasis was put on the comparison between two MiGPATs regarding subcellular localization and TAG synthesis function. A polyclonal and monoclonal antibody were separately prepared for MiGPAT1 and MiGPAT2, and the following immunogold labeling showed they were localized on chloroplast and endoplasmic reticulum (ER), respectively. This was further confirmed by the GFP fusion studies. Both MiGPATs were expressed heterologously in a GPATdeficient yeast mutant, and it was found that MiGPAT2 played key roles in the de novo TAG biosynthesis, whereas MiGPAT1 was more involved in the phospholipid formation, contributing to TAG production via an indirect way. Findings of the present study expanded our knowledge on functional and evolutionary relationships of GPAT members in M. incisa, suggesting the possibility of genetic manipulation of GPAT in this alga for enhanced TAG production.
资助项目	National Natural Science Foundation of China (NSFC)[31772821] ; Science and Technology Commission of Shanghai Municipality[20ZR1423900] ; NSFC-Shandong Joint Fund[U1706209] ; State Double First-Class Discipline Project of Aquaculture
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000632338300011
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/170323]
专题	中国科学院海洋研究所
通讯作者	Sun, Zheng; Zhou, Zhi-Gang
作者单位	1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Shanghai 200090, Peoples R China 4.Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China 5.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China
推荐引用方式 GB/T 7714	Sun, Li-Ping,Ouyang, Long-Ling,Bao, Hong,et al. Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa : Subcellular localization and role in triacylglycerol synthesis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,54:10.
APA	Sun, Li-Ping,Ouyang, Long-Ling,Bao, Hong,Liu, Jian-Guo,Sun, Zheng,&Zhou, Zhi-Gang.(2021).Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa : Subcellular localization and role in triacylglycerol synthesis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,54,10.
MLA	Sun, Li-Ping,et al."Comparison between two isoforms of glycerol-3-phosphate acyltransferase in microalga Myrmecia incisa : Subcellular localization and role in triacylglycerol synthesis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 54(2021):10.