Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates

doi:10.1038/s41929-019-0305-8

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates
	Li, XY ; Cao, YF ; Luo, K ; Sun, YZ ; Xiong, JR ; Wang, LC ; Liu, Z ; Li, J ; Ma, JY ; Ge, J
刊名	NATURE CATALYSIS
	2019
卷号	2 期号:8 页码:718-725
关键词	DYNAMIC KINETIC RESOLUTION TOTAL-ENERGY CALCULATIONS PRIMARY AMINES CHIRAL AMINES RACEMIZATION PD CATALYSTS PALLADIUM IMMOBILIZATION NANOPARTICLES
ISSN号	2520-1158
DOI	10.1038/s41929-019-0305-8
文献子类	期刊论文
英文摘要	Building a bridge between enzymatic and heterogeneous catalysis provides new cascade industrial processes for manufacturing. However, the reaction conditions of enzymatic and heterogeneous catalysis mutually cause deactivation of catalysts. Here, we overcame this challenge by developing a special protocol for the synthesis of hybrid catalysts. We utilized protein-polymer nanoconjugates as confined nanoreactors for the in situ synthesis of lipase-palladium (Pd) nanohybrids. The 0.8 nm Pd nanoparticles exhibited increased activity in racemization of (S)-1-phenylethylamine. At 55 degrees C, which matches the optimum temperature of lipase, the activity is more than 50 times that of commercial Pd/C. It was found that the Pd-O coordination in Pd subnanoclusters contributed to the high activity. In the dynamic kinetic resolutions of pharmaceutical intermediates (+/-)-1-phenylethylamine, (+/-)-1-aminoindan and (+/-)-1,2,3,4-tetrahydro-1-naphthylamine, the lipase-Pd nanohybrids displayed 7.6, 3.1 and 5.0 times higher efficiencies than the combination of commercial immobilized lipase Novozym 435 and Pd/C. The lipase-Pd nanohybrids can be reused without agglomeration and activity loss.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32082]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Tsinghua Univ, Dept Chem Engn, Key Lab Ind Biocatalysis, Minist Educ, Beijing, Peoples R China; 2.Fudan Univ, Dept Chem, Jiangwan Campus, Shanghai, Peoples R China; 3.Tsinghua Univ, Dept Chem, Beijing, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Li, XY,Cao, YF,Luo, K,et al. Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates[J]. NATURE CATALYSIS,2019,2(8):718-725.
APA	Li, XY.,Cao, YF.,Luo, K.,Sun, YZ.,Xiong, JR.,...&Zare, RN.(2019).Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates.NATURE CATALYSIS,2(8),718-725.
MLA	Li, XY,et al."Highly active enzyme-metal nanohybrids synthesized in protein-polymer conjugates".NATURE CATALYSIS 2.8(2019):718-725.