Star-PAP regulates tumor protein D52 through modulating miR-449a/34a in breast cancer

doi:10.1242/bio.045914

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Star-PAP regulates tumor protein D52 through modulating miR-449a/34a in breast cancer
	Duan, Aizhu 1,3; Kong, Lingmei 3; An, Tao 3; Zhou, Hongyu 3; Yu, Chunlei 2; Li, Yan 3
刊名	BIOLOGY OPEN
	2019-11-01
卷号	8 期号:11 页码:9
关键词	Star-PAP TPD52 miR-449a miR-34a Breast cancer
ISSN号	2046-6390
DOI	10.1242/bio.045914
通讯作者	Yu, Chunlei(yu_chunlei@foxmail.com) ; Li, Yan(liyan@mail.kib.ac.cn)
英文摘要	Tumor protein D52 (TPD52) is an oncogene amplified and overexpressed in various cancers. Tumor-suppressive microRNA-449a and microRNA-34a (miR-449a/34a) were recently reported to inhibit breast cancer cell migration and invasion via targeting TPD52. However, the upstream events are not clearly defined. Star-PAP is a non-canonical poly (A) polymerase which could regulate the expression of many miRNAs and mRNAs, but its biological functions are not well elucidated. The present study aimed to explore the regulative roles of Star-PAP in miR-449a/34a and TPD52 expression in breast cancer. We observed a negative correlation between the expression of TPD52 and Star-PAP in breast cancer. Overexpression of Star-PAP inhibited TPD52 expression, while endogenous Star-PAP knockdown led to increased TPD52. Furthermore, RNA immunoprecipitation assay suggested that Star-PAP could not bind to TPD52, independent of the 3'-end processing. RNA pull-down assay showed that Star-PAP could bind to 3'region of miR-449a. In line with these results, blunted cell proliferation or cell apoptosis caused by Star-PAP was rescued by overexpression of TPD52 or downregulation of miR-449a/34a. Our findings identified that Star-PAP regulates TPD52 by modulating miR-449a/34a, which may be an important molecular mechanism underlying the tumorigenesis of breast cancer and provide a rational therapeutic target for breast cancer treatment.
WOS研究方向	Life Sciences & Biomedicine - Other Topics
语种	英语
WOS记录号	WOS:000501353400007
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/70078]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Yu, Chunlei; Li, Yan
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.North Sichuan Med Coll, Sch Pharm, Inst Mat Med, Nanchong 637100, Sichuan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Duan, Aizhu,Kong, Lingmei,An, Tao,et al. Star-PAP regulates tumor protein D52 through modulating miR-449a/34a in breast cancer[J]. BIOLOGY OPEN,2019,8(11):9.
APA	Duan, Aizhu,Kong, Lingmei,An, Tao,Zhou, Hongyu,Yu, Chunlei,&Li, Yan.(2019).Star-PAP regulates tumor protein D52 through modulating miR-449a/34a in breast cancer.BIOLOGY OPEN,8(11),9.
MLA	Duan, Aizhu,et al."Star-PAP regulates tumor protein D52 through modulating miR-449a/34a in breast cancer".BIOLOGY OPEN 8.11(2019):9.