A peptide nucleic acid-regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles

doi:10.1007/s00604-020-04357-w

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	A peptide nucleic acid-regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles
	Gao, Tingting ; Xing, Shu ; Xu, Mengjia ; Fu, Pan ; Yao, Jiechen ; Zhang, Xiaokang ; Zhao, Yang ; Zhao, Chao
刊名	MICROCHIMICA ACTA
	2020
卷号	187 期号:7
关键词	QUANTUM DOTS GRAPHENE OXIDE PAIR SUBSTITUTIONS SENSING PLATFORM TRANSFER FRET PROBES SENSOR
DOI	10.1007/s00604-020-04357-w
英文摘要	A convenient fluorometric method was developed for specific determination of DNA based on peptide nuclei acid (PNA)-regulated fluorescence resonance energy transfer (FRET) between carbon dots (CDs) and gold nanoparticles (AuNPs). In this system, CDs that display lake blue fluorescence with excitation/emission maxima at 345/445 nm were used as fluorometric reporter, while AuNPs were used as fluorescence nanoquencher. A neutral PNA probe, which is designed to recognize the target DNA, was used as a coagulant to control the dispersion and aggregation of AuNPs. Without DNA, PNA can induce immediate AuNP aggregation, thus leading to the recovery of the FRET-quenched fluorescence emission of CDs. However, the addition of the complementary target DNA can protect AuNPs from being aggregated due to the formation of DNA/PNA complexes, which subsequently produces a high fluorescence quenching efficiency of CDs by dispersed AuNPs. Under optimized conditions, quantitative evaluation of DNA was achieved in a linear range of 5-100 nM with a detection limit of 0.21 nM. This method exhibited an excellent specificity towards fully matched DNA. In addition, the application of this assay for sensitive determination of DNA in cell lysate demonstrates its potential for bioanalysis and biodetection. A simple fluorometric biosensor for specific detection of DNA was developed based on peptide nuclei acid (PNA)-regulated fluorescence resonance energy transfer (FRET) between carbon dots (CDs) and gold nanoparticles (AuNPs).
学科主题	Chemistry
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20742]
专题	2020专题
作者单位	1.Xing, S 2.Zhao, C (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Cixi Inst Biomed Engn, Ningbo 315201, Peoples R China. 3.Zhao, Y (corresponding author), Ningbo Univ, Coll Sci & Technol, Ningbo 315212, Peoples R China.
推荐引用方式 GB/T 7714	Gao, Tingting,Xing, Shu,Xu, Mengjia,et al. A peptide nucleic acid-regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles[J]. MICROCHIMICA ACTA,2020,187(7).
APA	Gao, Tingting.,Xing, Shu.,Xu, Mengjia.,Fu, Pan.,Yao, Jiechen.,...&Zhao, Chao.(2020).A peptide nucleic acid-regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles.MICROCHIMICA ACTA,187(7).
MLA	Gao, Tingting,et al."A peptide nucleic acid-regulated fluorescence resonance energy transfer DNA assay based on the use of carbon dots and gold nanoparticles".MICROCHIMICA ACTA 187.7(2020).