Infrared Spectroscopic Study of Pepsin Secondary Structure Changes on Surfaces

doi:10.19756/j.issn.0253.3820.201189

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	Infrared Spectroscopic Study of Pepsin Secondary Structure Changes on Surfaces
	Guo Xiao-Mi ; Zhang Xiao-Xuan ; Chu Wu-Bo ; Jiang Nan ; Li He
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2020
卷号	48 期号:9 页码:1273-1278
关键词	FTIR BARE ATR
DOI	10.19756/j.issn.0253.3820.201189
英文摘要	Pepsin is an extremely important digestive enzyme in the stomach of mammals, which breaks down proteins in food by cutting off specific peptide bonds into amino acids. Pepsin is very sensitive to the pH of surroundings as a kind of acid protease, so the purpose of this experiment is to investigate the influence on the secondary structure of pepsin under various pH values and surfaces by Fourier transform infrared spectroscopy (FTIR) technology. The result shows that with the increase of pH, the percentage of beta-sheet increases and reaches maximum in pH 3 to 7 and decreases when the solution is basic. The percentage of random coil is opposite to the beta-sheet. When the ATR crystal surface is coated with TiO2, the percentage of beta-sheet is much greater than other secondary structures. Moreover, with the change of pH, the percentage of each secondary structure has no significant change. At the same time, quantitative analysis shows that the interaction between pepsin and TiO2 is beneficial to the adsorption of pepsin on surface. It indicates that the functional groups can also affect the conformation of pepsin on the surface.
学科主题	Chemistry
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20531]
专题	2020专题
作者单位	1.Li, H (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Marine Mat & Related Technol, Zhejiang Key Lab Marine Mat & Protect Technol,CAS, Ningbo 315201, Peoples R China. 2.Li, H (corresponding author), Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China.
推荐引用方式 GB/T 7714	Guo Xiao-Mi,Zhang Xiao-Xuan,Chu Wu-Bo,et al. Infrared Spectroscopic Study of Pepsin Secondary Structure Changes on Surfaces[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2020,48(9):1273-1278.
APA	Guo Xiao-Mi,Zhang Xiao-Xuan,Chu Wu-Bo,Jiang Nan,&Li He.(2020).Infrared Spectroscopic Study of Pepsin Secondary Structure Changes on Surfaces.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,48(9),1273-1278.
MLA	Guo Xiao-Mi,et al."Infrared Spectroscopic Study of Pepsin Secondary Structure Changes on Surfaces".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 48.9(2020):1273-1278.