A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.)

doi:10.1007/s00425-020-03355-9

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.)
	Ye, Jin ; Ding, Wona ; Chen, Yujie ; Zhu, Xinni ; Sun, Jiutong ; Zheng, Wenjuan ; Zhang, Botao ; Zhu, Shihua
刊名	PLANTA
	2020
卷号	251 期号:4
关键词	OXIDATIVE STRESS PROTEOMIC ANALYSIS CELL-DIVISION EXPRESSION GROWTH RESISTANCE BIOSYNTHESIS PROTEINS LEAVES GENE
DOI	10.1007/s00425-020-03355-9
英文摘要	Main conclusion Dysfunctional mutation of OsNDPK4 resulted in severe defects in root development of rice. However, the resistance of Osndpk4 against bacterial blight was significantly enhanced. Nucleoside diphosphate kinases (NDPKs) are an evolutionarily conserved family of important enzymes balancing the energy currency nucleoside triphosphates by catalyzing the transfer of their phosphate groups. The aim of this study was to elucidate the function of OsNDPK4 in rice. A dysfunctional rice mutant was employed to characterize the function of OsNDPK4. Its expression and subcellular localization were examined. The transcriptomic change in roots of Osndpk4 was analyzed by RNA-seq. The rice mutant Osndpk4 showed severe defects in root development from the early seedling stage. Further analysis revealed that meristematic activity and cell elongation were significantly inhibited in primary roots of Osndpk4, together with reduced accumulation of reactive oxygen species (ROS). Map-based cloning identified that the mutation occurred in the OsNDPK4 gene. OsNDPK4 was found to be expressed in a variety of tissues throughout the plant and OsNDPK4 was located in the cytosol. Osndpk4 showed enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo) and up-regulation of pathogenesis-related marker genes. In addition, transcriptomic analysis showed that OsNDPK4 was significantly associated with a number of biological processes, including translation, protein modification, metabolism, biotic stress response, etc. Detailed analysis revealed that the dysfunction of OsNDPK4 might reorchestrate energy homeostasis and hormone metabolism and signalling, resulting in repression of translation, DNA replication and cell cycle progression, and priming of biotic stress defense. Our results demonstrate that OsNDPK4 plays important roles in energy homeostasis, development process, and defense responses in rice.
学科主题	Plant Sciences
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/19884]
专题	2020专题
作者单位	1.Ding, WN 2.Zhang, BT (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Cixi Inst Biomed Engn, Ningbo 315201, Peoples R China. 3.Zhu, SH (corresponding author), Ningbo Univ, Coll Sci & Technol, Ningbo 315211, Peoples R China.
推荐引用方式 GB/T 7714	Ye, Jin,Ding, Wona,Chen, Yujie,et al. A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.)[J]. PLANTA,2020,251(4).
APA	Ye, Jin.,Ding, Wona.,Chen, Yujie.,Zhu, Xinni.,Sun, Jiutong.,...&Zhu, Shihua.(2020).A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.).PLANTA,251(4).
MLA	Ye, Jin,et al."A nucleoside diphosphate kinase gene OsNDPK4 is involved in root development and defense responses in rice (Oryza sativa L.)".PLANTA 251.4(2020).