Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein

doi:10.1021/acs.nanolett.9b02855

CORC > 计算技术研究所 > 中国科学院计算技术研究所 > 中国科学院计算技术研究所期刊论文 > 英文

	Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
	Zhang, Mingshu 7; Fu, Zhifei 7,8; Li, Changqing 7; Liu, Anyuan 1,7; Peng, Dingming 7,8; Xue, Fudong 7,8; He, Wenting 7; Gao, Shan 2,8; Xu, Fan 2; Xu, Dan 3
刊名	NANO LETTERS
	2020-04-08
卷号	20 期号:4 页码:2197-2208
关键词	Photoconvertible fluorescent protein live super-resolution microscopy Drosophila embryos Quick-SIMBA pcStar
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.9b02855
英文摘要	Low temporal resolution and limited photo-controllable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photo-convertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1-0.25 s) with large field-of-view (76 X 9.4 mu m(2) -76 X 31.4 mu m(2)) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures in Drosophila embryos and revealed a specific "parallel three-pillar" structure in the neuronal-glial cell junction, helping to elucidate glial cell "locking" and support of neurons during Drosophila embryogenesis.
资助项目	National Key R&D Program of China[2017YFA0505300] ; National Key R&D Program of China[2016YFA0501500] ; National Natural Science Foundation of China[31670870] ; National Natural Science Foundation of China[31870857] ; National Natural Science Foundation of China[31421002] ; National Natural Science Foundation of China[21778069] ; Chinese Academy of Sciences[XDB08030203] ; Project of Chinese Academy of Sciences-Peking University Leading Cooperation Team
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000526413400001
内容类型	期刊论文
源URL	[http://119.78.100.204/handle/2XEOYT63/14199]
专题	中国科学院计算技术研究所期刊论文_英文
通讯作者	Xu, Tao; Xu, Pingyong
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 3.Fuzhou Univ, Coll Biol Sci & Engn, Inst Life Sci, Fuzhou 350116, Peoples R China 4.Cent South Univ, Ctr Med Genet, Sch Life Sci, Changsha 410078, Hunan, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 6.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 7.Chinese Acad Sci, Inst Biophys, Key Lab RNA Biol, Beijing 100101, Peoples R China 8.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100149, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Mingshu,Fu, Zhifei,Li, Changqing,et al. Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein[J]. NANO LETTERS,2020,20(4):2197-2208.
APA	Zhang, Mingshu.,Fu, Zhifei.,Li, Changqing.,Liu, Anyuan.,Peng, Dingming.,...&Xu, Pingyong.(2020).Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein.NANO LETTERS,20(4),2197-2208.
MLA	Zhang, Mingshu,et al."Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein".NANO LETTERS 20.4(2020):2197-2208.