Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment

doi:10.1007/s11356-021-12746-8

CORC > 成都山地灾害与环境研究所 > 中国科学院水利部成都山地灾害与环境研究所 > 山地表生过程与生态调控重点实验室

	Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment
	Cheng, Jianhua; Tang, Xiangyu; Liu, Chen
刊名	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
	2021-02-08
页码	12
关键词	Animal manure Antibiotic-resistant genes Mobile genetic elements Bacterial community Soil resistome High-throughput qPCR
ISSN号	0944-1344
DOI	10.1007/s11356-021-12746-8
通讯作者	Tang, Xiangyu(xytang@imde.ac.cn)
英文摘要	The increasing emergence of antibiotic-resistant genes (ARGs) represents a global threat to human health. Land application of animal manure is known to contribute considerably to the propagation and dispersal of antibiotic resistance in agro-ecosystems. Yet, the primary determinants of the fate of the soil resistome remain obscure. In this study, a pot experiment was conducted to examine temporal changes in ARGs, mobile genetic elements (MGEs), and bacterial communities in a weakly developed loamy soil (an entisol known as calcareous purple soil) upon addition of pig or chicken manure. On the day of manure application, substantial increases in the diversity and relative abundance of ARGs were observed in soil amended with raw pig manure. At the same time, no obvious changes were observed for soil amended with chicken manure. Antibiotic resistance in pig manure-amended soils rapidly decreased over time to a level that was still higher than that of unamended soil at 100 days after manure application. The results of the Mantel test and Procrustes analysis indicated that ARG profiles in soil were significantly correlated with the structure of the bacterial phylogeny. Variation partitioning analysis further revealed that the bacterial community played a major role in regulating the temporal changes in ARGs in soil following manure application. Increased numbers and relative abundances of MGEs and their significant positive correlations with ARGs were observed, which suggest that a potential contribution from lateral gene transfer to the persistence and spread of ARGs should not be overlooked. Overall, our findings provide a better understanding of the mechanisms underlying the dynamics of ARGs in entisols following manure application and have practical implications for managing manure applications in entisols of the study area and other areas.
资助项目	National Key Research and Development Program of China[2016YFD0800203] ; Major Science and Technology Program of Sichuan Province[2018SZDZX0029] ; National Natural Science Foundation of China[42007361] ; National Natural Science Foundation of China[41771521] ; 135 Strategic Program of the CAS Institute of Mountain Hazards and Environment[SDS-135-1702]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000616157600012
资助机构	National Key Research and Development Program of China ; Major Science and Technology Program of Sichuan Province ; National Natural Science Foundation of China ; 135 Strategic Program of the CAS Institute of Mountain Hazards and Environment
内容类型	期刊论文
源URL	[http://ir.imde.ac.cn/handle/131551/55964]
专题	成都山地灾害与环境研究所_山地表生过程与生态调控重点实验室
通讯作者	Tang, Xiangyu
作者单位	Chinese Acad Sci, Inst Mt Hazards & Environm, Key Lab Mt Surface Proc & Ecol Regulat, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Jianhua,Tang, Xiangyu,Liu, Chen. Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2021:12.
APA	Cheng, Jianhua,Tang, Xiangyu,&Liu, Chen.(2021).Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,12.
MLA	Cheng, Jianhua,et al."Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021):12.