Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis

doi:10.1007/s11816-020-00627-4

	Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis
	Wang, Yanli 1; Xie, Tian 1; Zhang, Chunli 1; Li, Juanjuan 1; Wang, Zhi 2,3; Li, Hongbing 2,3; Liu, Xiping 1; Yin, Lina 2,3; Wang, Shiwen 2,3; Zhang, Suiqi 2,3
刊名	PLANT BIOTECHNOLOGY REPORTS
	2020-05-19
页码	12
关键词	Stomatal density StEPF2 Drought tolerance Potato iWUE
ISSN号	1863-5466
DOI	10.1007/s11816-020-00627-4
通讯作者	Deng, Xiping(dengxp@ms.iswc.ac.cn) ; Ke, Qingbo(qbke@nwafu.edu.cn)
英文摘要	Epidermal patterning factor 2 (EPF2) is a negative regulator of stomatal development, and is essential for plant growth, development, and environmental stress responses. However, the role of EPF2 in potato (Solanum tuberosum) has not been investigated to date. Here, we cloned and characterized the potato EPF2-like gene (StEPF2). StEPF2 is predominantly intercellular space localized and its transcripts were rhythmically expressed, and showed the highest expression in apical unexpanded leaves. Expression of StEPF2 was markedly down-regulated in response to abscisic acid and sodium chloride treatments; however, upon the application of polyethylene glycol, the expression of StEPF2 peaked at 4 h and then decreased gradually. Overexpression of StEPF2 in Arabidopsis (OE) substantially reduced stomatal density and photosynthetic rate, but had little effects on plant growth. Under drought stress, OE lines maintained higher photosynthetic rates, photosystem II efficiency, and instantaneous water use efficiency than wild-type (WT) plants. Moreover, OE lines showed less water loss and hydrogen peroxide accumulation in detached leaves compared with WT plants. Thus, our results suggest that StEPF2 acts as a negative regulator of stomatal development in potato, indicating that the role of EPF2 is conserved across plant species. Overall, StEPF2 represents an important target for the development of drought-tolerant potato cultivars via genetic engineering.
资助项目	Fundamental Research Funds for the Northwest AF University[2452017189] ; Ph.D. Programs Foundation of Ministry of Education of China[2452018005] ; CAS Light of West China Program[XAB2017B02] ; National Natural Science Foundation of China[31901332] ; S&T Promotion Project for the Northwest AF University[Z222021810] ; 111 Project of the Ministry of Education, China[B12007] ; [A314021402-2013]
WOS关键词	WATER-USE EFFICIENCY ; AGROBACTERIUM-MEDIATED TRANSFORMATION ; MODULATING STOMATAL DENSITY ; FLORAL DIP ; SECRETORY PEPTIDE ; PLANT-RESPONSES ; CLIMATE-CHANGE ; TUBER YIELD ; STRESS ; GROWTH
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000534203400001
资助机构	Fundamental Research Funds for the Northwest AF University ; Ph.D. Programs Foundation of Ministry of Education of China ; CAS Light of West China Program ; National Natural Science Foundation of China ; S&T Promotion Project for the Northwest AF University ; 111 Project of the Ministry of Education, China
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/159550]
专题	中国科学院地理科学与资源研究所
通讯作者	Deng, Xiping; Ke, Qingbo
作者单位	1.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China 3.Northwest A&F Univ, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanli,Xie, Tian,Zhang, Chunli,et al. Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis[J]. PLANT BIOTECHNOLOGY REPORTS,2020:12.
APA	Wang, Yanli.,Xie, Tian.,Zhang, Chunli.,Li, Juanjuan.,Wang, Zhi.,...&Ke, Qingbo.(2020).Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis.PLANT BIOTECHNOLOGY REPORTS,12.
MLA	Wang, Yanli,et al."Overexpression of the potato StEPF2 gene confers enhanced drought tolerance in Arabidopsis".PLANT BIOTECHNOLOGY REPORTS (2020):12.