The responses of soil nitrogen transformation to nitrogen addition are mainly related to the changes in functional gene relative abundance in artificial Pinus tabulaeformis forests

doi:10.1016/j.scitotenv.2020.137679

	The responses of soil nitrogen transformation to nitrogen addition are mainly related to the changes in functional gene relative abundance in artificial Pinus tabulaeformis forests
	Li, Jingjing 1; Wang, Guoliang 1,2; Yan, Benshuai 2; Liu, Guobin 1,2
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-06-25
卷号	723 页码:11
关键词	Nitrogen addition Nitrogen functional genes Net N transformation N2O emission Temperate forest soil
ISSN号	0048-9697
DOI	10.1016/j.scitotenv.2020.137679
通讯作者	Liu, Guobin(gbliu@ms.iswc.ac.cn)
英文摘要	The increase of soil nitrogen (N) availability may alter soil microbial community composition and the natural N cycle in forest ecosystems. However, the responses of soil microbial nitrogen functional genes (NFGs) to N addition and their consequent effect on the N-cycle processes are poorly understood. In this study, soil samples were collected from an artificial Pinus tabulaeformis forest located in Loess Plateau (China) to which N at four different concentrations was added (0 [N0], 3 [N3], 6 [N6], and 9 [N9] g N m(-2) y(-1)) for 4 years. We quantified the relative abundance of NFGs using functional gene microarray GeoChip 5.0 and determined net N transformation and N2O emission rates in a 14-day incubation experiment. The results showed that N-3 and N6 treatments did not significantly affect the total relative abundance and diversity of NFGs assemblage but significantly increased the relative abundance of specific genes for the NH3 cycle (ureC, nirA, and nrfA), and nitrification (amoA) and denitrification (norB). These positive effects were related to the increase in soil organic C, NO3--N, and microbial biomass C (MBC). N9 treatment significantly decreased the relative abundance of all NFGs, and this negative impact was correlated with reduced dissolved organic C and MBC. Moreover, N addition significantly changed net N nitrification, mineralization, and N2O emission rates, and NFGs explained the higher variances in the N transformation processes than soil properties. Specifically, ammonia-oxidizing archaea (amoA-AOB) and MBC were the key factors related to net N nitrification; ureC, nirK, and MBC were the key factors related to net N mineralization; and narG and nirS were the key factors related to N2O emission. Our results show that global N deposition may mainly influence N transformation processes by regulating the corresponding NFG relative abundance, thereby affecting the N cycle in forest soils. (C) 2020 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China[41671513] ; National Key Research and Development Program of China[2017YFC0504601]
WOS关键词	AMMONIA-OXIDIZING BACTERIA ; COMMUNITY STRUCTURE ; MICROBIAL BIOMASS ; N2O EMISSIONS ; POTENTIAL NITRIFICATION ; PHOSPHORUS ADDITIONS ; DENITRIFICATION ; FERTILIZATION ; ARCHAEA ; RATES
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000535898400014
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/159516]
专题	中国科学院地理科学与资源研究所
通讯作者	Liu, Guobin
作者单位	1.Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingjing,Wang, Guoliang,Yan, Benshuai,et al. The responses of soil nitrogen transformation to nitrogen addition are mainly related to the changes in functional gene relative abundance in artificial Pinus tabulaeformis forests[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,723:11.
APA	Li, Jingjing,Wang, Guoliang,Yan, Benshuai,&Liu, Guobin.(2020).The responses of soil nitrogen transformation to nitrogen addition are mainly related to the changes in functional gene relative abundance in artificial Pinus tabulaeformis forests.SCIENCE OF THE TOTAL ENVIRONMENT,723,11.
MLA	Li, Jingjing,et al."The responses of soil nitrogen transformation to nitrogen addition are mainly related to the changes in functional gene relative abundance in artificial Pinus tabulaeformis forests".SCIENCE OF THE TOTAL ENVIRONMENT 723(2020):11.