Synthetic Fertilizer Increases Denitrifier Abundance and Depletes Subsoil Total N in a Long-Term Fertilization Experiment

doi:10.3389/fmicb.2020.02026

	Synthetic Fertilizer Increases Denitrifier Abundance and Depletes Subsoil Total N in a Long-Term Fertilization Experiment
	Wang, Ying 1,2; Ji, Hongfei 1,2; Wang, Rui 1; Hu, Yaxian 1,2; Guo, Shengli 1,2
刊名	FRONTIERS IN MICROBIOLOGY
	2020-08-31
卷号	11 页码:11
关键词	denitrifier nitrifier gene abundance subsoil Chinese Loess Plateau
ISSN号	1664-302X
DOI	10.3389/fmicb.2020.02026
通讯作者	Wang, Ying(yingwang@nwsuaf.edu.cn)
英文摘要	Chronic synthetic nitrogen (N) application can result in a significant accumulation of nitrate in the subsoil, which could alter subsoil N cycle and subsequently affect subsoil N levels. To understand how elemental interactions affect the cycle and storage of subsoil N, we examined the soils receiving no fertilizer control (CK), 30-year applications of synthetic fertilizer (CF), and CF plus organic manure (CF + OM). The N cycling microbial groups and activity were investigated through analyzing abundance of bacteria, nitrifiers and denitrifiers, potential nitrification (PNA) and denitrification (DEA) rates in the topsoil (0-20 cm) and subsoil depths (20-80 cm). Compared with the CK, the CF application increased subsoil nitrate but reduced or did not change subsoil microbial biomass N and total N. Corresponding to the increased nitrate, the abundances of denitrifiers increased in the CF subsoils. By contrast, the abundances of nitrifiers increased in the CF topsoil. Significant correlation between the abundances of nitrifiers and soil PNA was found in the topsoil, while significant correlation was also found in the subsoil between the abundances ofnirS- and/ornirK-type denitrifiers and DEA. These results suggest that the depleted or less changed subsoil total N by CF application might be partly related to the enriched denitrifiers groups and the related potential activity. The contrasting responses of nitrifiers and denitrifiers in the CF subsoil indicate a decoupling of both processes. Our findings highlight that the leached nitrate by synthetic fertilizer addition not only occurs as an environmental risk causing groundwater contamination but may also alter the subsoil N cycle through the denitrifier groups.
资助项目	National Key Research and Development Program of China[2016YFD0200308] ; National Key Research and Development Program of China[2016YFD0800105] ; CAS 'Light of West China' Program for Introduced Talent in the West, National Natural Science Foundation of China[41301322] ; Chinese Universities Scientific Fund[2014YB055]
WOS关键词	AMMONIA-OXIDIZING BACTERIA ; NITROGEN ADDITION ; SOIL-PROFILE ; MICROBIAL COMMUNITIES ; VERTICAL-DISTRIBUTION ; NITRATE ACCUMULATION ; ORGANIC FERTILIZERS ; DEEP-SOIL ; CARBON ; IMPACT
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000570592600001
资助机构	National Key Research and Development Program of China ; CAS 'Light of West China' Program for Introduced Talent in the West, National Natural Science Foundation of China ; Chinese Universities Scientific Fund
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/156977]
专题	中国科学院地理科学与资源研究所
通讯作者	Wang, Ying
作者单位	1.Northwest A&F Univ, Key Lab Soil Eros & Dryland Farming Loess Plateau, Yangling, Shaanxi, Peoples R China 2.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ying,Ji, Hongfei,Wang, Rui,et al. Synthetic Fertilizer Increases Denitrifier Abundance and Depletes Subsoil Total N in a Long-Term Fertilization Experiment[J]. FRONTIERS IN MICROBIOLOGY,2020,11:11.
APA	Wang, Ying,Ji, Hongfei,Wang, Rui,Hu, Yaxian,&Guo, Shengli.(2020).Synthetic Fertilizer Increases Denitrifier Abundance and Depletes Subsoil Total N in a Long-Term Fertilization Experiment.FRONTIERS IN MICROBIOLOGY,11,11.
MLA	Wang, Ying,et al."Synthetic Fertilizer Increases Denitrifier Abundance and Depletes Subsoil Total N in a Long-Term Fertilization Experiment".FRONTIERS IN MICROBIOLOGY 11(2020):11.