IbINH positively regulates drought stress tolerance in sweetpotato

doi:10.1016/j.plaphy.2019.11.039

	IbINH positively regulates drought stress tolerance in sweetpotato
	Yang, Dongjing 2,4; Xie, Yiping 4; Sun, Houjun 4; Bian, Xiaofeng 1; Ke, Qingbo 5; Kim, Ho Soo 3; Ji, Chang Yoon 3; Jin, Rong 4; Wang, Wenbin 6; Zhang, Chengling 4
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2020
卷号	146 页码:403-410
关键词	Sweetpotato Invertase inhibitor Drought stress Sugars
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2019.11.039
通讯作者	Li, Zongyun(zongyunli@jsnu.edu.cn) ; Ma, Daifu(daifuma@126.com) ; Kwak, Sang-Soo(sskwak@kribb.re.kr)
英文摘要	Invertase inhibitor (INH) post-translationally regulates the activity of invertase, which hydrolyzes sucrose into glucose and fructose, and plays essential roles in plant growth and development. However, little is known about the role of INH in growth and responses to environmental challenges in sweetpotato. Here, we identified and characterized an INH-like gene (IbINH) from sweetpotato. IbINH belongs to the pectin methylesterase inhibitor super family. IbINH transcript was the most abundant in storage roots. IbINH mRNA levels were significantly up-regulated in response to drought, abscisic acid (ABA), salicyclic acid (SA) and jasmonic acid (JA) treatments. Overexpressing IbINH in sweetpotato (SI plants) led to the decrease of plant growth and the increase of drought tolerance, while down-regulation of IbINH (RI plants) by RNAi technology resulted in vigorous growth and drought sensitivity. Furthermore, sucrose was increased and hexoses was decreased in SI plants, but the opposite results were observed in RI plants. Moreover, higher levels of sugars were accumulated in SI plants in comparison to non-transgenic plants (NT plants) and RI plants during water deficit. In addition, ABA biosynthesis-involved and abiotic stress response-involved genes were prominently up-regulated in SI plants under drought stress. Taken together, these results indicate that IbINH mediates plant growth and drought stress tolerance in sweetpotato via induction of source-sink strength and ABA-regulated pathway.
资助项目	China Agriculture Research System[CARS-104315] ; Jiangsu Government Scholarship for Oversea Studies[JS-2014-120] ; Foundation of Xuzhou Academy of Agricultural Sciences[XM2017001] ; Fundamental Research Funds for the Northwest AF University[2452017189] ; KRIBB Initiative Program[KGM5371911] ; Postgraduate Innovation Project of Jiangsu Normal University[KYCX17-1620]
WOS关键词	CELL-WALL INVERTASE ; PECTIN METHYLESTERASE ; STRUCTURAL INSIGHTS ; SUCROSE METABOLISM ; ECTOPIC EXPRESSION ; PLANT INVERTASE ; SEED WEIGHT ; INHIBITOR ; SUGAR ; TOMATO
WOS研究方向	Plant Sciences
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000510528300038
资助机构	China Agriculture Research System ; Jiangsu Government Scholarship for Oversea Studies ; Foundation of Xuzhou Academy of Agricultural Sciences ; Fundamental Research Funds for the Northwest AF University ; KRIBB Initiative Program ; Postgraduate Innovation Project of Jiangsu Normal University
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/132467]
专题	中国科学院地理科学与资源研究所
通讯作者	Li, Zongyun; Ma, Daifu; Kwak, Sang-Soo
作者单位	1.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China 2.Jiangsu Normal Univ, Sch Life Sci, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China 3.KRIBB, Plant Syst Engn Res Ctr, 125 Gwahak Ro, Daejeon 34141, South Korea 4.Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Sweetpotato, Xuzhou Inst Agr Sci Jiangsu Xuhuai Dist, Xuzhou 221131, Jiangsu, Peoples R China 5.Northwest A&F Univ, Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shanxi, Peoples R China 6.Shanxi Agr Univ, Coll Life Sci, Taigu 030801, Shanxi, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dongjing,Xie, Yiping,Sun, Houjun,et al. IbINH positively regulates drought stress tolerance in sweetpotato[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2020,146:403-410.
APA	Yang, Dongjing.,Xie, Yiping.,Sun, Houjun.,Bian, Xiaofeng.,Ke, Qingbo.,...&Kwak, Sang-Soo.(2020).IbINH positively regulates drought stress tolerance in sweetpotato.PLANT PHYSIOLOGY AND BIOCHEMISTRY,146,403-410.
MLA	Yang, Dongjing,et al."IbINH positively regulates drought stress tolerance in sweetpotato".PLANT PHYSIOLOGY AND BIOCHEMISTRY 146(2020):403-410.