Application of X-ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion

doi:10.1002/jsfa.11316

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Application of X-ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion
	Chen, Peilin 1,2; Xu, Yanhong 1; Yang, Shuqi 1; Chang, Qing 1,2,3; Zheng, Baodong 1,2,3,4; Zhang, Yi 1,2,3,4; Hu, Xiaoke 5; Zeng, Hongliang 1,2,3,4
刊名	JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
	2021-05-27
页码	11
关键词	Porphyra haitanensis polysaccharide fractions separation and purification antioxidant capacity molecular weight
ISSN号	0022-5142
DOI	10.1002/jsfa.11316
通讯作者	Hu, Xiaoke(xkhu@yic.ac.cn) ; Zeng, Hongliang(zhlfst@fafu.edu.cn)
英文摘要	BACKGROUND The separation and purification of Porphyra haitanensis polysaccharide (PHP), and the determination of changes in molecular weight (Mw) and antioxidant capacity after in vitro digestion, were undertaken. RESULTS Analysis of two polysaccharide fractions (PHP0.5-1-UF and PHP1.0-1-UF) by various techniques showed that they were very pure sulfated polysaccharides without pigment or protein. PHP0.5-1-UF was filamentous or 'tape-like' sheets, whereas PHP1.0-1-UF had some filaments and large numbers of rounded aggregates. The Mw of PHP, PHP0.5-1-UF and PHP1.0-1-UF was 2.06 x 10(6) (+/- 2.02%), 6.68 x 10(6) (+/- 3.17%), and 1.14 x 10(6) (+/- 3.44%) (g mol(-1)), respectively. After in vitro digestion, the Mw of PHP, PHP0.5-1-UF, and PHP1.0-1-UF decreased. Their antioxidant capacities were markedly higher than before digestion, especially PHP0.5-1-UF and its digestion products, which might be related to the reductions in Mw. CONCLUSION These findings provide a greater understanding of the separation and purification of sulfated polysaccharides and the influence of digestion on biological activity. They also contribute to the practical application of sulfated polysaccharides in functional foods. (c) 2021 Society of Chemical Industry.
资助项目	Scientific Research Foundation of the Graduate School of Fujian Agriculture and Forestry University[1122yb065] ; Support Project for Distinguished Young Scholars of Fujian Agriculture and Forestry University[xjq201714] ; Program for Leading Talent in Fujian Provincial University[660160190] ; Program for New Century Excellent Talents in Fujian Province University[KLA18058A]
WOS关键词	SIMULATED GASTROINTESTINAL DIGESTION ; STRUCTURAL-CHARACTERIZATION ; FORTUNELLA-MARGARITA ; MOLECULAR-WEIGHT ; INHIBITORY-ACTIVITIES ; CHEMICAL-PROPERTIES ; ALPHA-GLUCOSIDASE ; DEGRADATION ; FERMENTATION ; EXTRACTION
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
WOS记录号	WOS:000654997500001
资助机构	Scientific Research Foundation of the Graduate School of Fujian Agriculture and Forestry University ; Support Project for Distinguished Young Scholars of Fujian Agriculture and Forestry University ; Program for Leading Talent in Fujian Provincial University ; Program for New Century Excellent Talents in Fujian Province University
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29303]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Hu, Xiaoke; Zeng, Hongliang
作者单位	1.Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou, Fujian, Peoples R China 2.Minist Educ, Engn Res Ctr Fujian, Fujian Taiwan Special Marine Food Proc & Nutr, Fuzhou, Peoples R China 3.Fujian Agr & Forestry Univ, Fujian Prov Key Lab Qual Sci & Proc Technol Speci, Fuzhou, Peoples R China 4.Fujian Agr & Forestry Univ, China Ireland Int Cooperat Ctr Food Mat Sci & Str, Fuzhou, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Chen, Peilin,Xu, Yanhong,Yang, Shuqi,et al. Application of X-ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,2021:11.
APA	Chen, Peilin.,Xu, Yanhong.,Yang, Shuqi.,Chang, Qing.,Zheng, Baodong.,...&Zeng, Hongliang.(2021).Application of X-ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,11.
MLA	Chen, Peilin,et al."Application of X-ray diffraction and energy dispersive spectroscopy in the isolation of sulfated polysaccharide from Porphyra haitanensis and its antioxidant capacity under in vitro digestion".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021):11.