Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China

doi:10.1007/s00343-020-0022-9

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中国科学院牟平海岸带环境综合试验站

	Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China
	Peng Saijun 1,4; Liu Qingqing 1,4; Wang Lei 1,4; Sun Tingting 1,4; Shiganova, Tamara 3; Dong Zhijun 2,4
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-10-29
页码	8
关键词	Aurelia coerulea medusae ephyrae 16S rRNA gene analyzes genetic differentiation genetic variability
ISSN号	2096-5508
DOI	10.1007/s00343-020-0022-9
通讯作者	Dong Zhijun(zjdong@yic.ac.cn)
英文摘要	Aurelia spp. ephyrae have been reported to form blooms in sea cucumber aquaculture ponds in the Bohai and Yellow Seas. To identify the species, we carried out a genetic analysis of Aurelia spp. ephyrae and medusae based on mitochondrial 16S rRNA gene. Samples of four Aurelia sp. ephyrae populations were collected in sea cucumber aquaculture ponds and samples of four Aurelia sp. medusae populations were collected in coastal waters. Using a BLASTn search, we found that both the ephyrae collected in the aquaculture ponds and medusae collected in coastal waters belong to Aurelia coerulea. Seventeen haplotypes were recovered from the 16S rRNA gene. The overall haplotype diversity and nucleotide diversity of the 166 A. coerulea individuals were 0.686 and 0.329%, respectively, indicating high haplotype diversity and low nucleotide diversity. Moreover, the haplotype diversity of ephyrae populations were generally lower than that of medusae populations with close sampling points. The genetic differentiation between ephyrae populations collected in the sea cucumber aquaculture ponds and A. coerulea medusae collected in coastal waters was not significant, suggesting the ephyrae populations in the sea cucumber culture ponds were part of the same genetic group as the medusae populations in the coastal waters. Phylogeographic analysis of the 16S rRNA region revealed that there was no significant correlation between the haplotypes and the geographic distribution of populations. Pairwise fixation index values showed significant genetic differentiation and limited gene flow between A. coerulea population of Weifang and other locations.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050301] ; Special Exchange Program from the Chinese Academy of Sciences ; National Natural Science Foundation of China[41576152] ; National Natural Science Foundation of China[41876138] ; Instrument Developing Project of the Chinese Academy of Sciences[YJKYYQ20180047] ; Key Research and Development Program of Yantai[2018ZHGY073]
WOS关键词	JELLYFISH BLOOMS ; MOON JELLYFISH ; CNIDARIA ; SCYPHOZOA ; AURITA ; THREAT ; RDNA
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
WOS记录号	WOS:000585027200005
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; Special Exchange Program from the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Key Research and Development Program of Yantai
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28314]
专题	中国科学院牟平海岸带环境综合试验站烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Dong Zhijun
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Russian Acad Sci, Shirshov Inst Oceanol, Nakhimovskyi Pr 36, Moscow 117997, Russia 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Peng Saijun,Liu Qingqing,Wang Lei,et al. Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020:8.
APA	Peng Saijun,Liu Qingqing,Wang Lei,Sun Tingting,Shiganova, Tamara,&Dong Zhijun.(2020).Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,8.
MLA	Peng Saijun,et al."Molecular identification and population differentiation of Aurelia spp. ephyrae in sea cucumber aquaculture ponds of northern China".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2020):8.