Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals

doi:10.5004/dwt.2020.24924

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals
	Lv, Yingchun 1,2; Zhang, Yang 2,3; Song, Jiawei 2,3; Xu, Gang 1,2
刊名	DESALINATION AND WATER TREATMENT
	2020-02-01
卷号	177 页码:131-138
关键词	Alkaline phosphatase Kinetic characteristics Heavy metal Swan Lake
ISSN号	1944-3994
DOI	10.5004/dwt.2020.24924
通讯作者	Xu, Gang(gxu@yic.ac.cn)
英文摘要	Phosphatase-catalyzed hydrolysis of organic phosphorus is an important pathway for providing bioavailable phosphorus to aquatic organisms. In this paper, a eutrophic water body was used to investigate how phosphorus and heavy metal ions affect alkaline phosphatase activity (APA) and its kinetic properties. The results show that phosphate (PO43-), hexametaphosphate ((PO3)(6)(6-)), pyrophosphate (P2O74-), and beta-glycerophosphate (C3H4O6P2-) inhibit APA and the inhibition effects increased with increasing concentration at the range of 0.01-1 mmol L-1. Addition of heavy metal ions, that is, Co2+, Pb2+, and Cr6+ at concentrations of 1 mmol L-1, increased the APA 3.1-fold, 2.3-fold, and 2.7-fold, respectively. The Cu2+, Zn2+, and Ni2+ exhibited an inhibitory effect on APA in water. For 1 mmol L-1 Cu2+, the APA deceased by 37.7% relative to the control. As the concentrations of Cu2+, PO43-, (PO3)(6)(6-), and P2O74- increased, the V-max of the alkaline phosphatase (AP) remained stable and K-m increased, indicating that these ions are competitive inhibitors of AP. In addition, V-max/K-m decreased with increasing concentrations of Cu2+, PO43-, (PO3)(6)(6-), P2O74-, and C3H4O6P2-, demonstrating the decreased catalytic efficiency of the AP. In summary, phosphorus and heavy metal significantly affect conversion of organic phosphorus into bioavailable inorganic phosphorous by changing the APA in eutrophic water bodies.
资助项目	National Natural Science Foundation of China[41573120] ; National Natural Science Foundation of China[U1806215] ; Key Deployment Project of the Chinese Academy of Sciences[KFZD-SW-112] ; Science and Technology Service Network Initiative[KFJ-STS-ZDTP-023]
WOS关键词	DISSOLVED ORGANIC PHOSPHORUS ; ENZYMES ; IMPACT
WOS研究方向	Engineering ; Water Resources
语种	英语
WOS记录号	WOS:000544993600013
资助机构	National Natural Science Foundation of China ; Key Deployment Project of the Chinese Academy of Sciences ; Science and Technology Service Network Initiative
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28631]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Xu, Gang
作者单位	1.Qufu Normal Univ, Coll Geog & Tourism, Rizhao 276826, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Yingchun,Zhang, Yang,Song, Jiawei,et al. Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals[J]. DESALINATION AND WATER TREATMENT,2020,177:131-138.
APA	Lv, Yingchun,Zhang, Yang,Song, Jiawei,&Xu, Gang.(2020).Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals.DESALINATION AND WATER TREATMENT,177,131-138.
MLA	Lv, Yingchun,et al."Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals".DESALINATION AND WATER TREATMENT 177(2020):131-138.