SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing

doi:10.1016/j.talanta.2020.121397

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing
	Bi, Liyan 2; Wang, Xiao 2; Cao, Xiaowei 3; Liu, Luying 2; Bai, Congcong 2; Zheng, Qingyin 2; Choo, Jaebum 5; Chen, Lingxin 1,2,4
刊名	TALANTA
	2020-12-01
卷号	220 页码:8
关键词	Au@AgNR SERS nanotag Antibiotic susceptibility testing Minimum inhibitory concentration Principal component analysis
ISSN号	0039-9140
DOI	10.1016/j.talanta.2020.121397
通讯作者	Bi, Liyan(liyan_bi@163.com) ; Choo, Jaebum(jbchoo@cau.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	There is a challenge to obtain an ultrasensitive and rapid approach to detect bacteria and identify resistance. As a powerful bioanalytical tool, surface-enhanced Raman scattering (SERS) in bacterial detection have attracted increasing attentions. Herein, we developed a SERS-active Au@Ag core-shell nanorod (Au@AgNR) tag platform for ultrasensitive bacteria detection and antibiotic-susceptibility testing (AST). The platform established that surface enhanced Raman scattered Rhodamine 6G (R6G) absorption at 1517 cm(-1) had a good linearity (RI = 3865 + 193logC; R-2 = 0.97) with logarithm of E. coli concentration over a range of 10(7)-10(2) CFU (colony forming unit)/mL with limit of detection as low 10(2) CFU/mL. When E. coli was exposed to ampicillin at minimum inhibitory concentration (MIC, 4 mu g/mL), Raman spectroscopy showed the obvious variation between ampicillin-susceptible E. coli (Amp(-)-E. coli) and the ampicillin-resistant E. coli (Amp(+)-E. coli). Combined with principal component analysis (PCA) statistical analysis, the Raman intensity variation mentioned above allows to obtain rapid antibiotic resistance testing (<3.5 h). In addition, E.coli spiked into blood from C57BL/6 mice can be identified clearly, indicating the potential for point-of-care diagnostics.
资助项目	National Natural Science Foundation of China[21605005] ; National Natural Science Foundation of China[81530030] ; National Natural Science Foundation of China[81873697] ; National Natural Science Foundation of China[21976209] ; National Research Foundation of Korea[2019R1A2C3004375] ; National Research Foundation of Korea[2018M3A7B4071203] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Taishan Scholar Project Special Funding[ts20190962]
WOS关键词	ENHANCED RAMAN-SCATTERING ; SENSITIVE DETECTION ; GOLD ; IDENTIFICATION ; SPECTROSCOPY ; DESIGN ; PROBE ; ARRAY
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000571477400006
资助机构	National Natural Science Foundation of China ; National Research Foundation of Korea ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28396]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Bi, Liyan; Choo, Jaebum; Chen, Lingxin
作者单位	1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 2.Binzhou Med Univ, Transformat Otol & Neurosci Ctr, Yantai 264003, Peoples R China 3.Yangzhou Univ, Med Coll, Inst Translat Med, Yangzhou 225001, Jiangsu, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 5.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
推荐引用方式 GB/T 7714	Bi, Liyan,Wang, Xiao,Cao, Xiaowei,et al. SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing[J]. TALANTA,2020,220:8.
APA	Bi, Liyan.,Wang, Xiao.,Cao, Xiaowei.,Liu, Luying.,Bai, Congcong.,...&Chen, Lingxin.(2020).SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing.TALANTA,220,8.
MLA	Bi, Liyan,et al."SERS-active Au@Ag core-shell nanorod (Au@AgNR) tags for ultrasensitive bacteria detection and antibiotic-susceptibility testing".TALANTA 220(2020):8.