Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models | |
He, Na3; Wang, Yude1; Huang, Yan1,2; Chen, Lingxin2; Wang, Xiaoyan1,2; Lv, Changjun1; Yue, Shouwei3 | |
刊名 | JOURNAL OF MATERIALS CHEMISTRY B |
2020-11-21 | |
卷号 | 8期号:43页码:9899-9905 |
ISSN号 | 2050-750X |
DOI | 10.1039/d0tb01969k |
通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Lv, Changjun(lucky_lcj@sina.com) ; Yue, Shouwei(shouweiy@sdu.edu.cn) |
英文摘要 | Acute lung injury (ALI) is a diffuse inflammatory pulmonary damage caused by excessive ROS that break the coordination of normal physiological structures and functions. Hypochlorous acid (HOCl), one kind of ROS, is a hopeful biological marker for inflammation-related diseases. Therefore, the excessive generation of HOCl might be a significant reason for oxidative injury in ALI. Herein, we developed a fluorescent probe, namely BCy-HOCl, for quantitatively monitoring and visualizing HOCl in living cells and in vivo. The probe BCy-HOCl displayed a significant fluorescence signal enhancement towards HOCl with excellent selectivity and sensitivity. The variation of HOCl in the ALI cell model and ALI mouse model was evaluated with BCy-HOCl to clarify the relationship between ALI and HOCl. Our results verified that the HOCl levels conspicuously increased with the severity of the ALI. Thus, HOCl is likely to play a crucial part in the process of ALI, which will probably provide a new strategy for its treatment. |
资助项目 | National Nature Science Foundation of China[81573393] ; National Nature Science Foundation of China[21804010] ; National Nature Science Foundation of China[21976209] ; Science and Technology Innovation Development Plan of Yantai of China[2020MSGY113] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Research Initiation Fund of Binzhou Medical University[BY2020KYQD01] ; Taishan Scholar Project Special Funding[ts20190962] |
WOS关键词 | RESPIRATORY-DISTRESS-SYNDROME ; IMAGING BASAL ; LIVE-CELL ; MITOCHONDRIA ; HCLO ; LYSOSOME |
WOS研究方向 | Materials Science |
语种 | 英语 |
WOS记录号 | WOS:000588549200006 |
资助机构 | National Nature Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/28335] |
专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
通讯作者 | Chen, Lingxin; Lv, Changjun; Yue, Shouwei |
作者单位 | 1.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Shandong Univ, Qilu Hosp, Cheelo Coll Med, Rehabil Ctr, Jinan 250100, Peoples R China |
推荐引用方式 GB/T 7714 | He, Na,Wang, Yude,Huang, Yan,et al. Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models[J]. JOURNAL OF MATERIALS CHEMISTRY B,2020,8(43):9899-9905. |
APA | He, Na.,Wang, Yude.,Huang, Yan.,Chen, Lingxin.,Wang, Xiaoyan.,...&Yue, Shouwei.(2020).Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models.JOURNAL OF MATERIALS CHEMISTRY B,8(43),9899-9905. |
MLA | He, Na,et al."Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models".JOURNAL OF MATERIALS CHEMISTRY B 8.43(2020):9899-9905. |
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