Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography

doi:10.1007/s10811-020-02208-y

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography
	Zang, Fan 1,2; Qin, Song 1,3; Ma, Chengbo 1,2; Li, Wenjun 1,3; Lin, Jian 2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2020-08-03
页码	8
关键词	Pyropia yezoensis Rhodophyta R-phycoerythrin R-phycocyanin Purification Membrane chromatography
ISSN号	0921-8971
DOI	10.1007/s10811-020-02208-y
通讯作者	Li, Wenjun(wjli@yic.ac.cn) ; Lin, Jian(linjian3384@163.com)
英文摘要	R-phycoerythrin and R-phycocyanin are the main light-harvesting pigment-protein complexes inPyropia yezoensis. They have bright colors and a high degree of fluorescence, and are now widely used in biomedicine, food, and cosmetic industries. Therefore, membrane chromatography was used to isolate and purify phycobiliprotein fromP. yezoensis. Algal cells were disrupted by repeated freeze-thaw cycles. Then ammonium sulfate was added into the crude phycobiliprotein extract, so that the saturation of ammonium sulfate in the solution reached 20%, 30%, and 40% in sequence. At 30% and 40% ammonium sulfate saturation, precipitates of R-phycoerythrin and R-phycocyanin mixture in different proportions were obtained. The precipitates were ultra-filtrated to remove the salt, and then, the retentate was taken for membrane chromatographic treatment. In the membrane chromatographic treatment, salt-sensitive PVDF (polyvinyl difluoride) membranes were used for the follow-up experiments. By adjusting the concentration of ammonium sulfate, the membrane system changed the binding strength of various proteins, and then separated different types of phycobiliproteins. The final products of R-phycoerythrin (A(lambda max)/A(280)purity 4.25, yield 45%) and R-phycocyanin (two purity grades: purity 5.8, yield 14.70% and purity 4.18, yield 39.89%) were obtained and then characterized by spectrophotometric and fluorometric measurements and SDS-PAGE. However, the large-scale production of R-phycocyanin and R-phycoerythrin remains a bottleneck as the column chromatography is difficult to be applied for industrial production. This method avoids the problem of easy clogging of column chromatography and can be useful for mass-production of R-phycoerythrin and R-phycocyanin in the future based on the laboratory results.
资助项目	National Natural Science Foundation of China[41906109] ; Yantai key RD projects[2019XDHZ101] ; Yantai key RD projects[2020MSGY084]
WOS关键词	B-PHYCOERYTHRIN ; MARINE MACROALGAE ; PURIFICATION ; EXTRACTION ; PHYCOBILIPROTEINS
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000555414000002
资助机构	National Natural Science Foundation of China ; Yantai key RD projects
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28568]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室中国科学院烟台海岸带研究所
通讯作者	Li, Wenjun; Lin, Jian
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.Yintai Univ, Coll Life Sci, Yantai 264005, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zang, Fan,Qin, Song,Ma, Chengbo,et al. Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:8.
APA	Zang, Fan,Qin, Song,Ma, Chengbo,Li, Wenjun,&Lin, Jian.(2020).Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography.JOURNAL OF APPLIED PHYCOLOGY,8.
MLA	Zang, Fan,et al."Preparation of high-purity R-phycoerythrin and R-phycocyanin fromPyropia yezoensisin membrane chromatography".JOURNAL OF APPLIED PHYCOLOGY (2020):8.