Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production

doi:10.1039/c9ra04365a

	Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production
	Miao, Changlin 1,2,3; Li, Huiwen 1,2,3; Zhuang, Xinshu 1,2,3; Wang, Zhongming 1,2,3; Yang, Lingmei 1,2,3; Lv, Pengmei 1,2,3,4; Luo, Wen 1,2,3
刊名	RSC ADVANCES
	2019-09-22
卷号	9 期号:51 页码:29665-29675
DOI	10.1039/c9ra04365a
通讯作者	Yang, Lingmei(yanglm@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn)
英文摘要	In this work, porous cross-linked enzyme aggregates (p-CLEAs) were synthesized by the in situ co-precipitation method using CaCO3 microparticles as templates. The preparation procedure involved the immobilization of crude lipase as CLEAs via precipitation with ammonium sulfate and entrapping these lipase molecules into the CaCO3 templates, followed by DTT (dithiothreitol)-induced assembly of lipase molecules to form lipase microparticles (lipase molecules were assembled into microparticles internally using disulfide bonds within the lipase molecules as the molecular linkers and stimulated by dithiothreitol); finally, the removal of CaCO3 templates was performed by EDTA to form pores in CLEAs. The scanning electron microscopy analysis of p-CLEAs showed a porous structure. p-CLEAs showed obvious improvement in thermal stability (after incubation at 65 degrees C, p-CLEAs lipase retained 86% relative activity, while free lipase retained only 33.67%) and pH stability (p-CLEAs relative activity was over 90% while for free lipase, the relative activity ranged from 72% to 89% from pH 6 to 9) than free lipase and could hold relatively high activity retention without activity loss at 4 degrees C for more than six months. The application of p-CLEAs in producing biodiesel showed a higher degree of conversion. The conversion of fatty acid methyl ester (FAME) was 89.7%; this value was higher by approximately 7.4% compared to that of the conventional CLEAs under the optimized conditions of a methanol-oil molar ratio of 6 : 1, with a p-CLEAs lipase dose of 20% and water content of 3% at 45 degrees C for 24 h. The FAME conversion remained greater than 70% even after reusing the p-CLEAs lipase for 8 reactions. The results demonstrated that the p-CLEAs lipase is suitable for applications in the preparation of biodiesel.
资助项目	National Key RD Program[2017YFD0601003] ; National Nature Science Foundation of China[21506217] ; Natural Science Foundation of Guangdong Province[2016A030308004] ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences[Y707j91001]
WOS关键词	ENZYME AGGREGATES CLEAS ; IMMOBILIZATION ; STABILITY ; OIL ; MICROSPHERES ; PARTICLES ; PAPAIN
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000487364400022
资助机构	National Key RD Program ; National Nature Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/25643]
专题	中国科学院广州能源研究所
通讯作者	Yang, Lingmei; Lv, Pengmei
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou 510640, Guangdong, Peoples R China 4.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China
推荐引用方式 GB/T 7714	Miao, Changlin,Li, Huiwen,Zhuang, Xinshu,et al. Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production[J]. RSC ADVANCES,2019,9(51):29665-29675.
APA	Miao, Changlin.,Li, Huiwen.,Zhuang, Xinshu.,Wang, Zhongming.,Yang, Lingmei.,...&Luo, Wen.(2019).Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production.RSC ADVANCES,9(51),29665-29675.
MLA	Miao, Changlin,et al."Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production".RSC ADVANCES 9.51(2019):29665-29675.