养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探

doi:10.16441/j.cnki.hdxb.20170139

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探
	缪栋 ; 苏蕾 ; 张倩倩 ; 包卫洋 ; 龚骏
刊名	中国海洋大学学报(自然科学版)
	2018-06-15
卷号	48 期号:08 页码:68-76
关键词	藻类爆发石莼萱藻特异性引物孢子配子 ITS rDNA 实时荧光定量PCR
ISSN号	1672-5174
DOI	10.16441/j.cnki.hdxb.20170139
其他题名	Development and Application of ITS-rDNA Targeted Real-Time Quantitative PCR Assays for Detecting and Quantifying the Spores/Gamates of Harmful Marine Macroalgae
产权排序	(1)扬州大学环境科学与工程学院 ; (2)中国科学院烟台海岸带研究所
英文摘要	Marine macroalgal blooms in coastal eutrophic waters affect the physiology and ecology of the cultured organisms, causing massive death of these animals sometimes.Knowing the quantity and variation of the algal spores or gamates in the aquatic systems is crucial in understanding the biological and ecological mechanisms underlying Algal outbreaks and in developing strategies of prevention. In this study, we newly designed specific PCR primers targeting the internal transcript region(ITS) of the ribosomal RNA genes(rDNA) of three macroalgal species,Ulva cf.linza,Ulva sp.,and Scytosiphon lomentaria, which all are known to be potentially blooming species in mariculture ponds. The specificity of these primers was verified in silico, in vitro and in situ. Real-time quantitative PCR (qPCR)assays with these primers were developed and verified,showing a detection limitation as low as 200~400ITS copy numbers of the algal genomic DNA.The newly developed assays were further applied to quantify the spores/ga Mates of S.lomentariain surface waters of a farming pond for sea cucumbers. The quantification of the spores of S.lomentariaduring a 16-month period (from December of 2014 to March of 2016)showed that the ITS rDNA copy number of this species was relatively High in December of 2015,which was in coincidence with the observed blooming of the species in March of 2016, suggesting a link between the high spore abundance in the winter and the bloom of the macroalga in the early spring of next year.
语种	中文
CSCD记录号	CSCD:6276853
资助机构	烟台市科技发展计划项目#2014ZH073 ; 烟台市科技发展计划项目#2015ZH074 ; 中国科学院战略性先导科技专项#XDA11020702 ; 国家自然科学基金项目#41522604 ; 国家自然科学基金项目#31301867
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24697]
专题	海岸带生物资源高效利用研究与发展中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.扬州大学环境科学与工程学院; 2.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	缪栋,苏蕾,张倩倩,等. 养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探[J]. 中国海洋大学学报(自然科学版),2018,48(08):68-76.
APA	缪栋,苏蕾,张倩倩,包卫洋,&龚骏.(2018).养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探.中国海洋大学学报(自然科学版),48(08),68-76.
MLA	缪栋,et al."养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探".中国海洋大学学报(自然科学版) 48.08(2018):68-76.