一株单环刺螠致病弧菌的分离鉴定、生长特性研究及药敏分析

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	一株单环刺螠致病弧菌的分离鉴定、生长特性研究及药敏分析
	于士翔 ; 纪元 ; 李新 ; 刘峰 ; 刘芳华
刊名	海洋科学
	2019-07-15
卷号	43 期号:07 页码:112-121
关键词	单环刺螠弧菌分离鉴定生长特性药敏试验
ISSN号	1000-3096
其他题名	Isolation,identification,characterization,and sensitivity analysis of gut pathogenic Vibrio of Urechis unicinctus
产权排序	(1) 中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室 ; (2) 中国科学院大学 ; (3) 中国农业大学烟台研究院
文献子类	期刊论文
英文摘要	The larvae of the diseased intestinal pathogenic bacterium Urechis unicinctus(3000 to 4000 kg) were isolated and identified, and their growth characteristics and drug sensitivities were evaluated to provide a treatment basis for diseases that occur in an artificial culture of the larva. Isolation and purification, biochemical identification, 16 S rRNA gene sequence analysis, artificial resumption infection, and growth curve assay as well as studies on optimum growth temperature, pH, and salt content were conducted on the pathogenic bacteria isolated from human intestines. The K–B disk diffusion method was applied for drug sensitivity testing, whereas ofloxacin was used for security detection testing. For pathogenic Vibrio, the optimum growth temperature, pH, and salt content were 30℃, 6.0, and 35, respectively. Drug sensitivity tests demonstrated that Vibrio is highly sensitive to chloramphenicol, carbenicillin, ofloxacin, and ceftriaxone; moderately sensitive to minocycline, gentamicin, kanamycin, cefazolin, and tetracycline; but resistant to azithromycin, neomycin, vancomycin, rifampicin, erythromycin, clindamycin, and amoxicillin. Security detection tests revealed that although ofloxacin exerted a clear antibacterial effect, it did not harm U. unicinctus. Based on the results of the drug sensitivity test, Vibrio-infected U. unicinctus can be effectively treated using targeted antibiotics. These experimental results could provide a theoretical basis and treatment pattern for the prevention and treatment of U. unicinctus in artificial cultures.
语种	中文
资助机构	国家自然科学基金(91751112,41573071) ; “科学”号高端用户项目(KEXUE2018G01) ; 山东省自然科学杰出青年基金(JQ201608) ; “泰山学者”青年专家计划(tsqn 20161054) ; 山东省重点研发计划(公益类专项,2018GHY115028)~~
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25199]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室; 2.中国科学院大学; 3.中国农业大学烟台研究院
推荐引用方式 GB/T 7714	于士翔,纪元,李新,等. 一株单环刺螠致病弧菌的分离鉴定、生长特性研究及药敏分析[J]. 海洋科学,2019,43(07):112-121.
APA	于士翔,纪元,李新,刘峰,&刘芳华.(2019).一株单环刺螠致病弧菌的分离鉴定、生长特性研究及药敏分析.海洋科学,43(07),112-121.
MLA	于士翔,et al."一株单环刺螠致病弧菌的分离鉴定、生长特性研究及药敏分析".海洋科学 43.07(2019):112-121.