酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定
	杨贵兰 ; 秦松 ; 王晓艳 ; 李文军
刊名	食品工业科技
	2019-10-08
卷号	41 期号:05 页码:163-168+174
关键词	阿拉斯加鳕鱼皮降压肽酶解法 ACE抑制率分子量分布氨基酸含量
ISSN号	1002-0306
其他题名	Extraction Optimization and Structure Identification of Anti-hypertensive Peptide from Alaskan Cod by Enzymatic Method
产权排序	(1) 滨州医学院药学院 ; (2) 中国科学院烟台海岸带研究所
文献子类	期刊论文
英文摘要	The technology for preparation of anti-hypertensive peptides from Alaska cod skins with optimum enzyme was optimized by using the index of ACE inhibitory rate in vitro. In this study,the optimal enzymatic hydrolysis process of anti-hypertensive peptides from Alaskan cod was determined by single-factor and orthogonal experiments,and the amino acid content and molecular weight distribution range of anti-hypertensive peptides were investigated. The results showed that the trypsin had the best hydrolyzing capacity,the ACE inhibition rate reached a constant value of 68.48%. And the optimum hydrolysis conditions were as follows:Hydrolysis temperature 40 ℃,the ratio of enzyme to substrate 3%,the ratio of solid to liquid 1∶7,pH7,hydrolysis time 2 h. Under these conditions,the ACE inhibition rate was 79.62%,content was 83%,the relative molecular weight below 500 Da was 70.74%,respectively. The optimized extraction process of anti-hypertensive peptides from Alaskan cod was feasible and reasonable. The results of this study could provide a reference for further investigation of food-derived anti-hypertensive peptides.
语种	中文
资助机构	高值化海洋低聚肽系列产品制备技术的研究与产业化项目 ; 烟台市“十三五”海洋经济创新发展示范项目(YHCX-SW-L-201705) ; 烟台市重点研发项目(Y933021011)
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25195]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室中国科学院烟台海岸带研究所
作者单位	1.滨州医学院药学院; 2.中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	杨贵兰,秦松,王晓艳,等. 酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定[J]. 食品工业科技,2019,41(05):163-168+174.
APA	杨贵兰,秦松,王晓艳,&李文军.(2019).酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定.食品工业科技,41(05),163-168+174.
MLA	杨贵兰,et al."酶法制备阿拉斯加鳕鱼降压肽的工艺优化及其产物的结构鉴定".食品工业科技 41.05(2019):163-168+174.