Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain

doi:10.1016/j.envpol.2019.113703

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain
	Min, Jun ; Xu, Lingxue ; Fang, Suyun ; Chen, Weiwei ; Hu, Xiaoke
刊名	ENVIRONMENTAL POLLUTION
	2020-03
卷号	258 页码:113703
关键词	BACILLUS-THERMOGLUCOSIDASIUS A7 AROMATIC HALOGENATED DBPS DISINFECTION BY-PRODUCTS P-NITROPHENOL PSEUDOMONAS-STUTZERI GENE-CLUSTER 2,4,6-TRICHLOROPHENOL DEGRADATION CATABOLISM PATHWAY CONTAMINATED SOIL BIODEGRADATION
DOI	10.1016/j.envpol.2019.113703
产权排序	[Min, Jun ; Xu, Lingxue ; Fang, Suyun ; Chen, Weiwei ; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China ; [Min, Jun ; Fang, Suyun ; Chen, Weiwei ; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China ; [Min, Jun ; Hu, Xiaoke] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China ; [Xu, Lingxue] Yantai Univ, Coll Life Sci, Yantai, Peoples R China
文献子类	Article
英文摘要	2,6-Dichloro-4-nitrophenol (2,6-DCNP) is an emerging chlorinated nitroaromatic pollutant, and its fate in the environment is an important question. However, microorganisms with the ability to utilize 2,6-DCNP have not been reported. In this study, Cupriavidus sp. CNP-8 having been previously reported to degrade various halogenated nitrophenols, was verified to be also capable of degrading 2,6-DCNP. Biodegradation kinetics assay showed that it degraded 2,6-DCNP with the specific growth rate of 0.124 h(-1), half saturation constant of 0.038 mM and inhibition constant of 0.42 mM. Reai-time quantitative PCR analyses indicated that the hnp gene cluster was involved in the catabolism of 2,6-DCNP. The hnpA and hnpB gene products were purified to homogeneity by Ni-NTA chromatography. Enzymatic assays showed that HnpAB, a FAD-dependent two-component monooxygenase, converted 2,6-DCNP to 6-chlorohydroxyquinol with a K-m of 3.9 +/- 1.4 mu M and a k(cat)/K-m of 0.12 +/- 0.04 mu M-1 min(-1). As the oxygenase component encoding gene, hnpA is necessary for CNP-8 to grow on 2,6-DCNP by gene knockout and complementation. The phylogenetic analysis showed that the hnp cluster originated from the cluster involved in the catabolism of chlorophenols rather than nitrophenols. To our knowledge, CNP-8 is the first bacterium with the ability to utilize 2,6-DCNP, and this study fills a gap in the microbial degradation mechanism of this pollutant at the molecular, biochemical and genetic levels. Moreover, strain CNP-8 could degrade three chlorinated nitrophenols rapidly from the synthetic wastewater, indicating its potential in the bioremediation of chlorinated nitrophenols polluted environments. (C) 2019 Elsevier Ltd. All rights reserved.
WOS关键词	BACILLUS-THERMOGLUCOSIDASIUS A7 ; AROMATIC HALOGENATED DBPS ; DISINFECTION BY-PRODUCTS ; P-NITROPHENOL ; PSEUDOMONAS-STUTZERI ; GENE-CLUSTER ; 2,4,6-TRICHLOROPHENOL DEGRADATION ; CATABOLISM PATHWAY ; CONTAMINATED SOIL ; BIODEGRADATION
语种	英语
WOS记录号	WOS:000519655100130
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31600085] ; State's Key Project of Research and Development Plan [2016YFC1402300] ; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013] ; Youth Innovation Promotion Association Program of the Chinese Academy of Sciences ; Yantai Science and Technology Project [2017ZH092]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25079]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China; 4.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Min, Jun,Xu, Lingxue,Fang, Suyun,et al. Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain[J]. ENVIRONMENTAL POLLUTION,2020,258:113703.
APA	Min, Jun,Xu, Lingxue,Fang, Suyun,Chen, Weiwei,&Hu, Xiaoke.(2020).Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain.ENVIRONMENTAL POLLUTION,258,113703.
MLA	Min, Jun,et al."Microbial degradation kinetics and molecular mechanism of 2,6-dichloro-4-nitrophenol by a Cupriavidus strain".ENVIRONMENTAL POLLUTION 258(2020):113703.