A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571

doi:10.1094/MPMI-03-19-0059-R

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571
	Liu, XL ; Xie, ZH ; Wang, YX ; Sun, Y ; Dang, XX ; Sun, HS
刊名	MOLECULAR PLANT-MICROBE INTERACTIONS
	2019-09
卷号	32 期号:9 页码:1134-1147
关键词	genetics and gene regulation molecular signaling Rhizobium-legume symbiosis
ISSN号	0894-0282
DOI	10.1094/MPMI-03-19-0059-R
产权排序	[Liu, Xiaolin ; Xie, Zhihong ; Wang, Yixuan ; Sun, Yu ; Dang, Xiaoxiao] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China ; [Liu, Xiaolin ; Wang, Yixuan ; Sun, Yu ; Dang, Xiaoxiao] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Sun, Haishuan] Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China
文献子类	Article
英文摘要	Azorhizobium caulinodans ORS571 can induce nodule formation on the roots and the stems of its host legume, Sesbania rostrata. Plant exudates are essential in the dialogue between microbes and their host plant and, in particular, amino acids can play an important role in the chemotactic response of bacteria. Histidine, arginine, and aspartate, which are the three most abundant amino acids present in S. rostrata seed exudates, behave as chemoattractants toward A. caulinodans. A position-specific-iterated BLAST analysis of the methyl-accepting chemotaxis proteins (MCPs) (chemoreceptors) in the genome of A. caulinodans was performed. Among the 43 MCP homologs, two MCPs harboring a dCache domain were selected as possible cognate amino acid MCPs. After analysis of relative gene expression levels and construction of a gene-deleted mutant strain, one of them, AZC_0821 designed as TlpH, was confirmed to be responsible for the chemotactic response to the three amino acids. In addition, it was found that these three amino acids can also influence chemotaxis of A. caulinodans independently of the chemosensory receptors, by being involved in the increase of the expression level of several the and fla genes involved in the chemotaxis pathway and flagella synthesis. Thus, the contribution of amino acids present in seed exudates is directly related to the role as chemoattractants and indirectly related to the role in the regulation of expression of key genes involved in chemotaxis and motility. This "dual role" is likely to influence the formation of biofilms by A. caulinodans and the host root colonization properties of this bacterium.
WOS关键词	BACILLUS-AMYLOLIQUEFACIENS ; ROOT COLONIZATION ; ORGANIC-ACIDS ; CHEMORECEPTORS ; CLONING ; SYSTEM ; GENE ; IDENTIFICATION ; NODULATION ; RESISTANCE
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
语种	英语
WOS记录号	WOS:000484540600009
资助机构	NSFC-Shandong Joint Fund Key Projects [U1806206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31570063, 31870020] ; Shandong Key Research and Development Program [2017GSF17129] ; Shandong Key Scientific and Technological Innovation Program [2017CXGC0303] ; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences [+CXGC2016B10]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24868]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China
推荐引用方式 GB/T 7714	Liu, XL,Xie, ZH,Wang, YX,et al. A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2019,32(9):1134-1147.
APA	Liu, XL,Xie, ZH,Wang, YX,Sun, Y,Dang, XX,&Sun, HS.(2019).A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571.MOLECULAR PLANT-MICROBE INTERACTIONS,32(9),1134-1147.
MLA	Liu, XL,et al."A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571".MOLECULAR PLANT-MICROBE INTERACTIONS 32.9(2019):1134-1147.