Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin
Liu, YM; Cui, YL; Chen, J; Qin, S; Chen, G
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
2019-12
卷号44页码:UNSP 101679
关键词Synechocystis sp. PCC6803 Astaxanthin beta-Carotene ketolase (bkt) Carotenoid hydroxylase (crtR-B) Metabolic engineering
ISSN号2211-9264
DOI10.1016/j.algal.2019.101679
产权排序[Liu, Yaming ; Cui, Yulin ; Chen, Jun ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China ; [Liu, Yaming] Univ Chinese Acad Sci, Beijing 101418, Peoples R China ; [Chen, Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China ; [Chen, Gao] Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
文献子类Article
英文摘要Astaxanthin is a carotenoid with wide applications because of its strong antioxidant properties. It is mainly produced from the green alga Haematococcus pluvialis, but large-scale production remains a challenge. To provide another source, here, Synechocystis sp. PCC6803 was adopted as the chassis cell, and its carotenoid biosynthesis pathway was genetically extended to produce astaxanthin. The beta-carotene ketolase gene (bkt) and carotenoid hydroxylase gene (crtR-B) from H. pluvialis, which wild-type Synechocystis sp. PCC6803 lacks, were optimized based on the codon preference of Synechocystis sp. PCC6803 and heterologously expressed in that bacterium to create a strain capable of producing astaxanthin. The Synechocystis sp. PCC6803 mutant synthesized 4.81 +/- 0.06 mg astaxanthin per gram of cells (dry weight) after nitrogen starvation for 14 days. More than 30% of the total astaxanthin was in the free form, and 82.61% was the strong antioxidant 3S, 3'S-astaxanthin isoform. These results show the industrial application potential of metabolically-engineered Synechocystis sp. PCC6803 for astaxanthin production.
WOS关键词HAEMATOCOCCUS-PLUVIALIS ; CRTR-B ; MICROALGAE ; ACCUMULATION ; CYANOBACTERIA ; CAROTENOIDS ; ISOMERS
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000498792900039
资助机构National Key PD Program [2016YFE0106700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876188] ; Natural Science Foundation of Shandong Province, ChinaNatural Science Foundation of Shandong Province [ZR2018ZB0210, ZR2016CM48] ; Key Research and Development Program of Shandong [2018GSF121019, 2019GSF107098] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences [YIC755031013] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-ZDTP-023]
内容类型期刊论文
源URL[http://ir.yic.ac.cn/handle/133337/24805]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 101418, Peoples R China;
3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China;
4.Shandong Prov Key Lab Acad Crop Genet Improvement, Jinan 250100, Shandong, Peoples R China
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Liu, YM,Cui, YL,Chen, J,et al. Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44:UNSP 101679.
APA Liu, YM,Cui, YL,Chen, J,Qin, S,&Chen, G.(2019).Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44,UNSP 101679.
MLA Liu, YM,et al."Metabolic engineering of Synechocystis sp. PCC6803 to produce astaxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44(2019):UNSP 101679.
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