Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis

doi:10.1016/j.algal.2019.101697

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis
	Lin, B ; Cui, YL ; Yan, MY ; Wang, YC ; Gao, ZQ ; Meng, CX ; Qin, S
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2019-12
卷号	44 页码:UNSP 101697
关键词	Astaxanthin beta-Carotene hydroxylase beta-Carotenoid ketolase Chloroplast transformation Dunaliella viridis Haematococcus pluvialis
ISSN号	2211-9264
DOI	10.1016/j.algal.2019.101697
产权排序	[Lin, Bin ; Gao, Zhengquan ; Meng, Chunxiao] Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China ; [Cui, Yulin ; Wang, Yinchu ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China ; [Yan, Mingyan] Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Shandong, Peoples R China
文献子类	Article
英文摘要	Dunaliella viridis is a green microalga containing beta-carotene, which is the precursor of astaxanthin, the most active antioxidant in Haematococcus pluvialis. Two key enzymes of H. pluvialis, beta-carotene hydroxylase (CRTR-B) and beta-carotenoid ketolase (BKT), are required for converting beta-carotene to astaxanthin in D. viridis via the astaxanthin biosynthetic pathway. Considering the location of beta-carotene in the chloroplast of D. viridis, the two modified genes encoding BKT and CRTR-B in H. pluvialis were integrated via homologous recombination into the chloroplast genome, in this study. In the chloroplast, the homologous recombination vector pMD-bkt-crtr (16S-TrnA-atpA-bkt-crtR-B-rbcL-psbA-bar-TrnI-23S), bkt and crtR-B were regulated by the atpA promoter in a polycistron. The presence of astaxanthin in the D. viridis mutant expressing BKT and CRTR-B was verified using high performance liquid chromatography (HPLC), and the maximum content of total astaxanthin and canthaxanthin after high light induction were 77.5 +/- 7.7 and 50.1 +/- 0.8 mu g g(-1) in dry weight, respectively. Our results indicate that D. viridis can be used as a cell factory for astaxanthin production.
WOS关键词	HAEMATOCOCCUS-PLUVIALIS ; EXPRESSION ; GENES ; EXTRACTION ; BIOSYNTHESIS ; ACCUMULATION ; PURIFICATION ; SYSTEM ; SALINA
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
WOS记录号	WOS:000498792900017
资助机构	National Key PD Program [2016YFE0106700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876188] ; Shandong Provincial Natural Science Foundation, ChinaNatural Science Foundation of Shandong Province [ZR2018ZB0210] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences [YIC755031013] ; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose) [2018YYSP016] ; Key Research and Development Program of Shandong Province [2016GSF121030, 2017GSF21105]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24804]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China; 3.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Lin, B,Cui, YL,Yan, MY,et al. Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,44:UNSP 101697.
APA	Lin, B.,Cui, YL.,Yan, MY.,Wang, YC.,Gao, ZQ.,...&Qin, S.(2019).Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,44,UNSP 101697.
MLA	Lin, B,et al."Construction of astaxanthin metabolic pathway in the green microalga Dunaliella viridis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 44(2019):UNSP 101697.