Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus

doi:10.1264/jsme2.ME18065

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus
	Wang, Yaping ; Shi, Tiantian ; Huang, Guoqiang ; Gong, Jun
刊名	MICROBES AND ENVIRONMENTS
	2018-09-29
卷号	33 期号:3 页码:290-300
关键词	food source gut microbiome life cycle marine benthos mycobiome
ISSN号	1342-6311
DOI	10.1264/jsme2.ME18065
产权排序	[Wang, Yaping ; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China ; [Wang, Yaping] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Shi, Tiantian] South China Normal Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China ; [Huang, Guoqiang] Guangxi Inst Oceanol, Beihai 536000, Peoples R China ; [Gong, Jun] Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Campus, Zhuhai, Guangdong, Peoples R China
文献子类	Article
英文摘要	The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. Overall, we detected a wide range of eukaryotic phylotypes associated with the larvae of S. nudus and in the gut contents of both worms. These phylotypes included ciliates, diatoms, dinoflagellates, eustigmatophytes, placidids, oomycetes, fungi, nematodes, flatworms, seaweeds, and higher plants. Oomycetes were associated with the planktonic larvae of S. nudus. The composition of eukaryotic diets shifted greatly across the larval, juvenile, and adult stages of S. nudus, and among different gut sections in U unicinctus, reflecting lifestyle changes during the ontogeny of the peanut worm and progressive digestion in the spoon worm. Malassezia-like fungi were prevalent in mycobiomes. Epicoccum and Trichosporon-related phylotypes dominated mycobiomes associated with larval individuals and in the gut contents of adults, respectively. The gut mycobiome of S. nudus was successively characterized through the midgut, aspiratory intestines, hindgut, and rectum as having a high proportion of Climacodon-Rhizochaete, Ceriporiopsis, Cladosporium-Pseudomicrostroma, and Afalassezia-related species in the libraries. These results emphasize the dynamics of diets and gut mycobiomes in marine benthic animals.
WOS关键词	FACULTATIVE ANAEROBIC NANOFLAGELLATE ; WORM SIPUNCULUS-NUDUS ; FUNGAL COMMUNITIES ; SUIGETSUMONAS-CLINOMIGRATIONIS ; MICROBIAL EUKARYOTES ; ORGANIC-MATTER ; BACTERIA ; SEA ; DNA ; DIVERSITY
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
WOS记录号	WOS:000446256500008
资助机构	Key Research Program of Frontier Sciences of CAS [QYZDB-SSW-DQC013] ; NSFC project [41522604] ; Strategic Priority Research Program of CAS [XDA11020702]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24534]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Guangxi Inst Oceanol, Beihai 536000, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.South China Normal Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China; 5.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai Campus, Zhuhai, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yaping,Shi, Tiantian,Huang, Guoqiang,et al. Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus[J]. MICROBES AND ENVIRONMENTS,2018,33(3):290-300.
APA	Wang, Yaping,Shi, Tiantian,Huang, Guoqiang,&Gong, Jun.(2018).Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus.MICROBES AND ENVIRONMENTS,33(3),290-300.
MLA	Wang, Yaping,et al."Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus".MICROBES AND ENVIRONMENTS 33.3(2018):290-300.