Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples

doi:10.1007/s00343-018-6326-3

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	Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples
	Su Lei ; Zhang Qianqian ; Gong Jun
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2018-05
卷号	36 期号:3 页码:818-826
关键词	Ciliophora Peritrichia clone library internal transcribed spacer (ITS) rDNA specific PCR primers
ISSN号	2096-5508
DOI	10.1007/s00343-018-6326-3
产权排序	[Su Lei ; Zhang Qianqian ; Gong Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai 264003, Peoples R China ; [Su Lei ; Zhang Qianqian ; Gong Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Peritrich ciliates are highly diverse and can be important bacterial grazers in aquatic ecosystems. Morphological identifications of peritrich species and assemblages in the environment are time-consuming and expertise-demanding. in this study, two peritrich-specific PCR primers were newly designed to amplify a fragment including the internal transcribed spacer (ITS) region of ribosomal rDNA from environmental samples. The primers showed high specificity in silico, and in tests with peritrich isolates and environmental DNA. Application of these primers in clone library construction and sequencing yielded exclusively sequences of peritrichs for water and sediment samples. We also found the ITS1, ITS2, ITS, D1 region of 28S rDNA, and ITS+D1 region co-varied with, and generally more variable than, the V9 region of 18S rDNA in peritrichs. The newly designed specific primers thus provide additional tools to study the molecular diversity, community composition, and phylogeography of these ecologically important protists in different systems.
WOS关键词	BIOFILM COMMUNITIES ; SEQUENCE ALIGNMENT ; WATER-QUALITY ; DIVERSITY ; RNA ; VORTICELLIDAE ; CILIOPHORA ; PROTOZOA ; SYSTEM ; CHINA
WOS研究方向	Limnology ; Oceanography
语种	英语
WOS记录号	WOS:000438791100020
资助机构	National Natural Science Foundation of China [31572255, 41522604, 31301867] ; Strategic Priority Research Program of CAS [XDA11020702] ; Science and Technology Development Program of Yantai [2014ZH073]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24468]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Microbial Ecol & Matter Cycles, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Su Lei,Zhang Qianqian,Gong Jun. Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(3):818-826.
APA	Su Lei,Zhang Qianqian,&Gong Jun.(2018).Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(3),818-826.
MLA	Su Lei,et al."Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.3(2018):818-826.